Patents Assigned to Mirus Bio Corporation
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Patent number: 7439051Abstract: The present invention relates to compositions and methods for the selection and use of surface exposed epitopes. The present invention includes in vivo and in vitro phage peptide diplay methods for the identification and selection of peptides and peptide associated factors with desired properties (e.g., targeting specificity, stability, etc.). The present invention further provides methods and compositions for the isolation and identification of peptide-specific antibodies. The present invention also includes methods and compositions employing nuclear localization signals for enhanced nuclear transport and expression of DNA.Type: GrantFiled: March 8, 2004Date of Patent: October 21, 2008Assignee: Mirus Bio CorporationInventors: Alexander V. Sokoloff, Jon A. Wolff
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Patent number: 7435723Abstract: Disclosed is a system for providing in vivo delivery of polynucleotides to mammalian prostate cells using an intravascular administration route. The polynucleotides are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the polynucleotide to the cell.Type: GrantFiled: October 6, 2003Date of Patent: October 14, 2008Assignee: Mirus Bio CorporationInventors: James E. Hagstrom, Mark Noble, Julia Hegge, Vladimir G. Budker
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Patent number: 7396919Abstract: An ionic polymer is utilized in “recharging” (another layer having a different charge) a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The resulting recharged complex can be formed with an appropriate amount of positive or negative charge such that the resulting complex has the desired net charge.Type: GrantFiled: June 9, 1999Date of Patent: July 8, 2008Assignee: Mirus Bio CorporationInventors: Jon A. Wolff, Vladimir S. Trubetskoy, Aaron G. Loomis, Paul M. Slattum, Sean D. Monahan, James E. Hagstrom, Vladimir G. Budker
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Patent number: 7348453Abstract: Described are ortho carboxy phenol derived acetals and compositions containing ortho carboxy phenol derived acetals which are useful for delivering biologically active compounds to cells. The acetals can be used to reversibly link up to three different molecules and have rapid hydrolysis kinetics in conditions which are present in a cell as well as in vivo. Cleavage of the acetal enhances delivery of the biologically active compound.Type: GrantFiled: August 30, 2004Date of Patent: March 25, 2008Assignee: Mirus Bio CorporationInventors: David B. Rozema, Darren Wakefield
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Patent number: 7326780Abstract: Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.Type: GrantFiled: May 1, 2006Date of Patent: February 5, 2008Assignee: Mirus Bio CorporationInventors: Paul M. Slattum, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker
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Patent number: 7262056Abstract: We disclose compositions and processes for enhancing transposon mediated integration of a nucleic acid molecule into another target nucleic acid molecule. Integration by an integrator complex is enhanced by cationic reagents.Type: GrantFiled: November 8, 2002Date of Patent: August 28, 2007Assignee: Mirus Bio CorporationInventors: Christine Wooddell, Hans Herweijer, Jon A. Wolff
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Patent number: 7220400Abstract: Cyclic amidinium containing compounds and their methods of preparation are described. Compositions containing these compounds facilitate delivery of biologically active polymers to cells in vitro and in vivo.Type: GrantFiled: May 27, 2003Date of Patent: May 22, 2007Assignee: Mirus Bio CorporationInventors: Sean D. Monahan, Vladimir Trubetskoy
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Publication number: 20070105804Abstract: Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.Type: ApplicationFiled: October 10, 2006Publication date: May 10, 2007Applicant: MIRUS BIO CORPORATIONInventors: Jon Wolff, Julia Hegge, James Hagstrom, Vladimir Budker
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Patent number: 7214369Abstract: A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from ischemia. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.Type: GrantFiled: May 5, 2003Date of Patent: May 8, 2007Assignee: Mirus Bio CorporationInventors: Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom, Julia Hegge, Vladimir Subbotin
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Patent number: 7208314Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.Type: GrantFiled: February 26, 2002Date of Patent: April 24, 2007Assignee: Mirus Bio CorporationInventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
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Patent number: 7192605Abstract: The present invention relates to compositions and methods for transferring nucleic acids into cells in vitro and in vivo. The compositions comprise a transfection reagent and one or more detergents. In preferred embodiments, the compositions comprise delivery systems providing nucleic acid transfer complexes that transfect cells with high efficiency.Type: GrantFiled: May 31, 2002Date of Patent: March 20, 2007Assignee: Mirus Bio CorporationInventors: Hans Herweijer, Vladimir G. Budker
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Publication number: 20070054872Abstract: Endogenous gene regulation mechanisms together with microRNAs expressed in many organisms can be used to provide regulated or conditional expression of transgenes by placing an appropriate sequence, a microRNA binding site, within the transcribed gene. This microRNA-dependent transcription regulation can be further regulated using microRNA inhibitors.Type: ApplicationFiled: August 24, 2006Publication date: March 8, 2007Applicant: Mirus Bio CorporationInventors: Thomas Reppen, David Lewis
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Publication number: 20070048254Abstract: A method is provided for expanding the population of mature dendritic cells in an individual subject. Proper timing of sequential delivery of Flt3-L and GM-CSF provides improved expansion of mature dendritic cell populations over methods currently employed in the art. The expansion of mature dendritic cells can be used to enhance an immune response in the subject. The mature dendritic cells can also be isolated from the subject for use in biological research.Type: ApplicationFiled: August 24, 2006Publication date: March 1, 2007Applicant: MIRUS BIO CORPORATIONInventors: Zane Neal, Hans Herweijer
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Publication number: 20070036865Abstract: We describe pH-sensitive endosomolytic polymers, delivery particles containing pH-sensitive endosomolytic polymers. The described particles are capable of delivering polynucleotides to cells from the peripheral circulation with subsequent release from endosomes. The endosomolytic polymers are inactive outside the cell but disrupt membranes upon exposure to an acidified endosomal compartment.Type: ApplicationFiled: September 19, 2006Publication date: February 15, 2007Applicant: MIRUS BIO CORPORATIONInventors: David Rozema, Darren Wakefield, Jon Wolff, Vladimir Budker, Tatyana Budker, Sean Monahan, Vladimir Trubetskoy, James Hagstrom, Aaton Loomis, Paul Slattum
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Patent number: 7148205Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.Type: GrantFiled: June 20, 2003Date of Patent: December 12, 2006Assignee: Mirus Bio CorporationInventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
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Patent number: 7144869Abstract: Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.Type: GrantFiled: December 5, 2002Date of Patent: December 5, 2006Assignee: Mirus Bio CorporationInventors: Jon A. Wolff, Julia Hegge, James E. Hagstrom, Vladimir G. Budker
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Patent number: 7138382Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.Type: GrantFiled: July 15, 2003Date of Patent: November 21, 2006Assignee: Mirus Bio CorporationInventors: Jon A. Wolff, James E. Hagstrom, David B. Rozema, Sean D. Monahan, Vladimir G. Budker
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Patent number: 7101995Abstract: Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.Type: GrantFiled: May 28, 2002Date of Patent: September 5, 2006Assignee: Mirus Bio CorporationInventors: David L. Lewis, James E. Hagstrom, Hans Herweijer, Aaron G. Loomis, Sean D. Monahan, Jon A. Wolff
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Patent number: 7098032Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.Type: GrantFiled: January 28, 2005Date of Patent: August 29, 2006Assignee: Mirus Bio CorporationInventors: Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
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Patent number: 7098030Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.Type: GrantFiled: July 26, 2004Date of Patent: August 29, 2006Assignee: Mirus Bio CorporationInventors: David B. Rozema, Vladimir G. Budker, James E. Hagstrom, Vladimir Trubetskoy, Jon A. Wolff, Sean D. Monahan, Paul M. Slattum