Patents Assigned to Mission Bio, Inc.
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Patent number: 11781129Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a method for analyzing nucleic acids within a cell includes the steps of: (a) flowing individual cells together with a material capable of forming a polymer or microsphere that retains nucleic acids into a carrier fluid such that droplets are formed; (b) breaking the emulsion and collecting the microsphere hydrogels in an aqueous fluid; and (c) performing combinatorial labeling on the nucleic acids contained within the microspheres/hydrogels.Type: GrantFiled: October 21, 2019Date of Patent: October 10, 2023Assignee: MISSION BIO, INC.Inventor: Dennis Jay Eastburn
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Patent number: 11667954Abstract: Provided herein are methods and systems for detection of nucleic acids for single cell samples. As part of the detection, a unique step of normalization of different single cell samples is included.Type: GrantFiled: July 1, 2020Date of Patent: June 6, 2023Assignee: Mission Bio, Inc.Inventors: David Ruff, Pedro Mendez
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Patent number: 11365441Abstract: Provided herein are methods and systems for the simultaneous targeted detection and sequencing of DNA, RNA, and Protein. In typical embodiments, the DNA, RNA, and Proteins are detected, characterized, and sequenced using just a single mammalian cell. One embodiment of detecting and characterizing DNA, RNA, or protein from a mammalian cell includes encapsulating a single cell in a drop and performing a protease digest on the encapsulated cell drop, performing a reverse transcriptase reaction; performing a droplet merger with barcoding PCR reagents and barcoding beads; performing a PCR reaction to attach the cell barcodes to the DNA targeted amplicons, RNA targeted amplicons, and protein tag amplicons, where all amplicons from the same emulsion contain the same cell barcode; and detecting and characterizing a DNA, RNA, or protein amplicon by sequencing the cell barcode incorporated into each amplicon.Type: GrantFiled: May 22, 2020Date of Patent: June 21, 2022Assignee: Mission Bio, Inc.Inventors: Dalia Dhingra, Aik Ooi, Pedro Mendez, David Ruff
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Publication number: 20210277458Abstract: Provided herein are methods for detection and characterization of a target nucleic acid from a single cell. One embodiment is a method for detection of a BCR-ABL gene fusion in a nucleic acid sample from a single cell having or suspected of having a BCR-ABL fusion transcript. One preferred implementation of the invention includes providing a nucleic acid amplification primer set complementary to a target nucleic acid suspected of having a BCR-ABL fusion transcript. In some embodiments, one or both primers of the nucleic acid amplification primer set have a barcode identification sequence. Also provided are methods for the detection of an AML tumor, methods are used for the detection of a leukemia, for the detection of a myeloid leukemia, and to determine the prognosis of a patient suspected of having a BCR-ABL fusion transcript.Type: ApplicationFiled: April 2, 2020Publication date: September 9, 2021Applicant: Mission Bio, Inc.Inventors: Dalia Dhingra, David Ruff, Pedro Mendez, Aik Ooi
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Publication number: 20210246488Abstract: The disclosure relates to methods and compositions for evaluating emulsion uniformity. In an exemplary embodiment, the disclosure provides a method for evaluating a quality characteristic of a droplet. The method includes the steps of: encapsulating a plurality of polynucleotides in a droplet, wherein the plurality of polynucleotides comprises at least one species of oligonucleotide; tagging the polynucleotides with a label that identifies the polynucleotides as arising from the droplet; counting a number of species of oligonucleotide tagged with the label; and determining a quality characteristic of the droplet based on the number of species of oligonucleotide tagged with the label. The oligonucleotide may include a first nucleic acid segment and a second nucleic acid segment, wherein the first nucleic acid segment comprises a plurality of random nucleotides; and the second nucleic acid segment comprises a conserved region common to the plurality of oligonucleotides.Type: ApplicationFiled: August 14, 2018Publication date: August 12, 2021Applicant: Mission Bio, Inc.Inventors: Sebastian Treusch, Adam R. Sciambi, Maurizio Pellegrino, Dennis Jay Eastburn
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Publication number: 20200325522Abstract: Provided herein are methods for detection and characterization of a target nucleic acid from a single cell. Some embodiments highlight the capability of identifying the biologically relevant variants at the moment of the diagnosis, but also how the treatment positively select resistant cellular clones based on the mutation signature. This positions the Tapestriā¢ platform described herein as the only tool available to study how genetic variants co-exist and which combinations are sensitive and resistant to certain treatments.Type: ApplicationFiled: April 2, 2020Publication date: October 15, 2020Applicant: Mission Bio, Inc.Inventors: Pedro Mendez, Dalia Dhingra, David Ruff, Aik Ooi
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Publication number: 20200071692Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a method for analyzing nucleic acids within a cell includes the steps of: (a) flowing individual cells together with a material capable of forming a polymer or microsphere that retains nucleic acids into a carrier fluid such that droplets are formed; (b) breaking the emulsion and collecting the microsphere hydrogels in an aqueous fluid; and (c) performing combinatorial labeling on the nucleic acids contained within the microspheres/hydrogels.Type: ApplicationFiled: October 21, 2019Publication date: March 5, 2020Applicant: Mission Bio, Inc.Inventor: Dennis Jay Eastburn
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Patent number: 10501739Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a method for analyzing nucleic acids within a cell includes the steps of: (a) flowing individual cells together with a material capable of forming a polymer or microsphere that retains nucleic acids into a carrier fluid such that droplets are formed; (b) breaking the emulsion and collecting the microsphere hydrogels in an aqueous fluid; and (c) performing combinatorial labeling on the nucleic acids contained within the microspheres/hydrogels.Type: GrantFiled: October 24, 2018Date of Patent: December 10, 2019Assignee: Mission Bio, Inc.Inventor: Dennis Jay Eastburn
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Publication number: 20190329209Abstract: Disclosed herein are methods and compositions for the emulsification of solid supports in deformable gel beads. The methods and compositions provided herein may be used in microfluidic systems and devices. In some aspects of the disclosure, deformable gel beads containing solid supports may be paired with single cell entities. The methods and compositions provided herein may be suitable for single cell analysis, including, but not limited to, labeling single cells or components thereof for downstream analysis.Type: ApplicationFiled: June 13, 2017Publication date: October 31, 2019Applicant: Mission Bio, Inc.Inventors: Adam R. Sciambi, Dennis Jay Eastburn, Sebastian Treusch
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Publication number: 20190112595Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a method for analyzing nucleic acids within a cell includes the steps of: (a) flowing individual cells together with a material capable of forming a polymer or microsphere that retains nucleic acids into a carrier fluid such that droplets are formed; (b) breaking the emulsion and collecting the microsphere hydrogels in an aqueous fluid; and (c) performing combinatorial labeling on the nucleic acids contained within the microspheres/hydrogels.Type: ApplicationFiled: October 24, 2018Publication date: April 18, 2019Applicant: Mission Bio, Inc.Inventor: Dennis Jay Eastburn
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Publication number: 20190112655Abstract: The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a microfluidic apparatus is used to provide a rapid and cost-effective targeted genomic sequencing of thousands of cells in parallel. The targeted sequencing can be directed for residual disease detection.Type: ApplicationFiled: October 18, 2018Publication date: April 18, 2019Applicant: Mission Bio, Inc.Inventors: Dennis Jay Eastburn, Adam R. Sciambi, Maurizio Pellegrino