Abstract: An exo-enzyme having an ability to cut .beta.-(2.fwdarw.1) fructoside bond or a microorganism which produces the enzyme is allowed to act on a saccharide solution containing cyclic inulooligosaccharide in which fructose molecules are bonded through .beta.-(2.fwdarw.1) bond in a cyclic configuration, and other saccharides such as monosaccharide, disaccharide, linear oligosaccharide and inulin, and then the cyclic inulooligosaccharide is collected from an obtained saccharide solution. Thus cyclic inulooligosaccharide can be purified inexpensively at a high yield without using any solvent.

Abstract: A method of separating a liquid by pervaporation, which includes purifying a liquid to be treated by at least one pervaporation membrane module unit providing a supply line connected to the pervaporation membrane module unit and supplying the liquid to be treated to the membrane module unit, utilizing a withdrawing line for withdrawing the permeated fluid from the membrane module unit and a withdrawing line for withdrawing the non-permeated liquid, wherein the supply line is provided with a pressure pump for transporting the liquid and a heater, and providing the final withdrawing line for the non-permeated liquid with a pressure regulating valve.

Abstract: An apparatus for separating a liquid by pervaporation, which comprises at least one pervaporation membrane module unit for purifying a liquid to be treated, a supply line connected to said pervaporation membrane module unit for supplying the liquid to be treated to the membrane module unit, a withdrawing line for withdrawing the permeated fluid from the membrane module unit and a withdrawing line for withdrawing the non-permeated liquid, wherein said supply line is provided with a pressure pump for transporting the liquid and a heater, and the final withdrawing line for the non-permeated liquid is provided with a pressure regulating valve.

Abstract: The method of chromatographic separation comprises: a feed-withdrawal step in which, with liquid flow being permitted from the first to the third unit beds in the simulated moving bed, an eluant is fed into the first unit bed and the feed stock is fed into the third unit bed while, at the same time, part of the effluent from the first unit bed is withdrawn from the system as a fraction rich in component B and the effluent from the third unit bed is withdrawn from the system as a fraction rich in component C; a circulation stage in which, with liquid circulation being permitted through the simulated moving bed, the liquid in the simulated moving bed is moved downstream without liquid feed to or withdrawal from the simulated moving bed; and a withdrawal stage in which, with liquid flow being permitted from the second to the first unit beds, the eluant is fed to the second unit bed and the effluent from the first unit bed is withdrawn from the system as a fraction rich in component A; wherein, after the above th

Abstract: A method of separating a liquid mixture wherein the liquid mixture is heated; the heated liquid mixture is supplied to a pervaporation membrane module to separate a permeable component of the liquid; a portion of non-permeated liquid is circulated through a circulation pipe into a liquid mixture feeding pipe before a heater, and the remaining portion of the non-permeated liquid is extracted to the outside of the system, and wherein the temperature of the liquid in the liquid mixture feeding pipe in which the non-permeated liquid has been mixed with the liquid mixture or in the circulation pipe for the non-permeated liquid, is measured, and when the measured temperature is out of a predetermined range, new supply of the liquid mixture and/or extraction of the non-permeated liquid is stopped.

Abstract: A coupling device intended to prevent impurities such as dust and particles adhering to the end face of a solution supplying male pipe 2 from being mixed with a high purity chemical solution N, which will be supplied through the device, not to degrade the purity of the solution. It includes a check valve 7 arranged in that end portion of a solution passage 5 in the male pipe 2 which is inserted into a cleaning chamber 15, a valve pusher 44a arranged in that end portion of a solution passage 43 in a solution supplied female pipe 3 which is connected to the passage 5 in the male pipe 2 to push the check valve 7 to open the passage 5, a valve system for opening and closing the passage 43 when the female pipe 3 is reciprocated in the axial direction, and a check valve 51 located on the outlet side of a cleaning liquid passage 48.