Abstract: This device comprises: a casing with an upper surface including a sample-dripping portion receiving a liquid sample containing nucleic acid and being dripped from a nozzle; a reaction tube: outwardly projecting from an end of the casing; including a storage space therein; and being formed so as to be installed within a measurement apparatus; a filter carrying the nucleic acid contained in the liquid sample; a filter-supporting body stored within the casing to support the filter in a manner such that the filter is capable of taking: a contacting position wherein the filter contacts with the liquid sample right below the sample-dripping portion; and a reaction position wherein the filter is positioned within the storage space of the reaction tube; and absorbing material capable of taking: a press-attaching position wherein the absorbing material is press-attached to the filter in the contacting position so that the filter absorbs the liquid sample contacting therewith; and a separating position wherein the abso

Abstract: The present invention provides a method for detecting multiple target nucleic acids, the method making it possible to amplify a plurality of genes using one reaction vessel containing therein one type of reaction solution and further using a single label. Computation is performed in accordance with the following formulas for every specific cycle and/or every cycle during amplification reaction using a kit for detecting the multiple target nucleic acids. (Formula 1): f1[n]=fhyb.1[n]/fden.1[n], (Formula 1?): f2[n]=fhyb.2[n]/fden.2[n], (Formula 2): Fr[n]=(a?f2 [n])/(a?f1 [n]).fhyb.1 [n]: Fluorescence intensity value in elongation step of first target nucleic acid detecting step. The same hereinafter.

Abstract: Provided is a kit for detecting multiple target nucleic acids capable of simultaneously amplifying and detecting multiple genes by means of one reaction vessel containing one kind of reaction solution and one kind of labels. Solution may contain first target nucleic acid (10) and second target nucleic acid (20) each of which dissociates at denaturation temperature T0.

Abstract: This device comprises: a casing with an upper surface including a sample-dripping portion receiving a liquid sample containing nucleic acid and being dripped from a nozzle; a reaction tube: outwardly projecting from an end of the casing; including a storage space therein; and being formed so as to be installed within a measurement apparatus; a filter carrying the nucleic acid contained in the liquid sample; a filter-supporting body stored within the casing to support the filter in a manner such that the filter is capable of taking: a contacting position wherein the filter contacts with the liquid sample right below the sample-dripping portion; and a reaction position wherein the filter is positioned within the storage space of the reaction tube; and absorbing material capable of taking: a press-attaching position wherein the absorbing material is press-attached to the filter in the contacting position so that the filter absorbs the liquid sample contacting therewith; and a separating position wherein the abso

Abstract: The present invention provides a method capable of realizing pretreatment process of genetic screening according to a POCT mode. The method includes: making a sample, extraction liquid for extracting nucleic-acid contained in the sample, silica particles, and a filtering material contact with each other; making the filtering material carry composite material of the nucleic-acid and the silica particles thereon; and then delivering the filtering material to a nucleic-acid-amplifying process by means of reaction solution for amplifying nucleic-acid, wherein particle diameters of the silica particles and concentration of the silica particles in the reaction solution for amplifying nucleic-acid are set up within a predetermined range.

Abstract: Provided is a kit for detecting multiple target nucleic acids capable of simultaneously amplifying and detecting multiple genes by means of one reaction vessel containing one kind of reaction solution and one kind of labels. Solution may contain first target nucleic acid (10) and second target nucleic acid (20) each of which dissociates at denaturation temperature T0.

Abstract: There is provided a sample trituration vessel that even when the sample is relatively hard, enables triturating of the sample easily and with high efficiency. The sample trituration vessel includes a tubular body having an opening at one end and having a bottom part at the other end thereof. The tubular body is furnished with a rugged portion there-inside so as to be able to meet another rugged port formed on a trituration rod. The sample trituration vessel itself can exert shearing effect on the sample. The tubular body is formed of flexible material, and upon application of external force from outside of the tubular body, the tubular body is deformed, thereby breaking down the sample held within the tubular body.

Abstract: A catcher, having an immunological epitope, is fixed to a detection zone of a spreading layer. A marker, capable of easy detection, has an inmmunological epitope. The marker and bispecific antibodies are soluble so that they can move on the spreading layer. The bispecific antibodies include a first bispecific antibody, having specificity for the detectable material in the fluid sample and the marker, and a second bispecific antibody, having specificity for the detectable material in the fluid sample and the catcher. Pore sizes and particle diameters are set so that the reaction product in which the marking elements and the particles are bonded are caught at a catching section. The concentration of the marking elements and the particles are increased to improve detection sensitivity. The result is an inexpensive and simple detection apparatus being highly-sensitive for the immunological detection of a detectable material, without wasting valuable antibodies.