Abstract: An automated high-precision plunger pump for volumes of less than 1 microliter, which is used in the microscope-assisted micromanipulation of biological material, comprises a housing (1) and a pump head (8) that defines a working chamber (9) with a common or separate inlet and outlet opening (10), wherein the volume of the working chamber (9) can be altered by an axially movable plunger (7) that can be introduced into it. The plunger (7) is axially movable via a threaded spindle (5) or a spindle nut (6) arranged in threaded engagement on the threaded spindle (5), and the threaded spindle (5) and/or the spindle nut (6) can be rotationally driven and the respective other component is designed to be rotationally lockable.

Abstract: A method for the collision-free positioning of a micromanipulation tool relative to a sample carrier with the aid of a microscope, comprising the following steps: a) determining the focal position Zp of at least one point P on the surface of the sample carrier by focusing a microscope objective with a first numerical aperture NA1 on the at least one point; b) positioning the micromanipulation tool on the optical axis of the microscope; c) determining the focal position ZM of the micromanipulation tool by focusing the microscope objective or a second microscope objective with a second numerical aperture NA2 smaller than the first numerical aperture NA1 onto the tip of the micromanipulation tool; d) calculating the lowering distance ZA(P) taking into account a predetermined tolerance profile ?Z by the following equation: ZA(P)=ZM?Zp??Z where ?Z=?ZM+?Zp, wherein ?ZM and/or ?Zp is a predetermined tolerance in the determination of the position of the manipulation tool and sample carrier, respectively; and e) posit

Abstract: A method for the collision-free positioning of a micromanipulation tool relative to a sample carrier with the aid of a microscope, comprising the following steps: a) determining the focal position Zp of at least one point P on the surface of the sample carrier by focusing a microscope objective with a first numerical aperture NA1 on the at least one point; b) positioning the micromanipulation tool on the optical axis of the microscope; c) determining the focal position ZM of the micromanipulation tool by focusing the microscope objective or a second microscope objective with a second numerical aperture NA2 smaller than the first numerical aperture NA1 onto the tip of the micromanipulation tool; d) calculating the lowering distance ZA(P) taking into account a predetermined tolerance profile ?Z by the following equation: ZA(P)=ZM?Zp??Z where ?Z=?ZM+?Zp, wherein ?ZM and/or ?Zp is a predetermined tolerance in the determination of the position of the manipulation tool and sample carrier, respectively; and e) posit

Abstract: An automated high-precision plunger pump for volumes of less than 1 microliter, which is used in the microscope-assisted micromanipulation of biological material, comprises a housing (1) and a pump head (8) that defines a working chamber (9) with a common or separate inlet and outlet opening (10), wherein the volume of the working chamber (9) can be altered by an axially movable plunger (7) that can be introduced into it. The plunger (7) is axially movable via a threaded spindle (5) or a spindle nut (6) arranged in threaded engagement on the threaded spindle (5), and the threaded spindle (5) and/or the spindle nut (6) can be rotationally driven and the respective other component is designed to be rotationally lockable.

Abstract: A manipulation system for microscope applications comprises a micromanipulation tool (1) and a protective device (2) protecting the tool, wherein the tool (1) and the protective device (2) are movable relative to one another between a position protecting the tool in a contactless manner and a position releasing the tool for the manipulation.