Abstract: A method for three-dimensional microdissection for separating defined structures in the sub-millimeter range by cold laser ablation or multi-photon absorption, whereby exposure of the structures to be separated is performed using directional information in all spatial directions. Also, a 3D microdissection system for separating defined, three-dimensional structures from a sample, having: a control unit (5); an ablation chamber (1) with a sample holder, on which the sample to be processed is mounted and which is movable along a linear axis V and rotatable about a rotary axis R. The sample holder has positioning devices connected to the control unit. The positioning devices move the sample holder along another linear axis H and rotate it about rotary axis R, and a laser device (6) is introduced into the ablation chamber at least partially through a laser window (3) in the ablation chamber.

Abstract: A method for three-dimensional microdissection for the separation of defined structures in the sub-millimeter range by means of cold laser ablation or by means of multi-photon absorption, whereby exposure of the structures to be separated is performed using directional information in all spatial directions.