Abstract: A method for maintaining a cell in vitro that comprises the steps of: providing a cell of interest in cell culture, determining differential breadth of histone 3 trimethylated at lysine 4 (H3K4me3) modified regions and a differential broadness score for each protein-coding gene in said cell, determining a network score for each protein-coding gene in said cell based on the differential broadness score and interactions between the protein products of each protein-coding gene over at least one network, and determining a cell identity score for each protein-coding gene in said cell based on a combination of the differential broadness score and network scores. The cell identity scores are used to prioritise protein-coding genes thereby identifying cell identity genes for said cell, which encode factors associated with cell identity. The cell of interest is cultured in the presence of said factors, thereby maintaining the cell identity of the cell of interest.

Abstract: The present invention relates to methods and compositions, including kits for reprogramming a source cell to a cardiomyocyte, the method comprising increasing the protein expression of one or more transcription factors, or variants thereof, in the source cell, wherein the source cell is reprogrammed to exhibit at least one characteristic of a cardiomyocyte.

Abstract: The present invention relates to methods and compositions for reprogramming a source cell to produce a chondrocyte, the method comprising activating or increasing the protein expression of one or more transcription factors, or variants thereof, in the source cell.