Abstract: Methods of detecting the absence or presence of a micro-organism in a sample comprising: contacting the sample with a nucleic acid molecule which acts as a substrate for nucleic acid modifying activity of the micro-organism in the sample, incubating the thus contacted sample under conditions suitable for nucleic acid modifying activity; and specifically determining the absence or presence of a modified nucleic acid molecule resulting from the action of the nucleic acid modifying activity on the substrate nucleic acid molecule to indicate the absence or presence of the micro-organism. Corresponding kits are also provided.
Type:
Grant
Filed:
June 20, 2022
Date of Patent:
September 5, 2023
Assignee:
Momentum Bioscience Limited
Inventors:
Matthew A. Crow, Helen V. Bennett, Daniel S. Wratting, William H. Mullen
Abstract: Methods for detecting a fungal/yeast infection in a sample, comprising: performing a nucleic acid amplification reaction to amplify the ILV3 gene of fungi/yeast; and detecting the amplification product to determine whether the sample contains a fungal/yeast infection. Corresponding primers, probes and kits are also provided.
Abstract: Methods of detecting the absence or presence of a micro-organism in a sample comprising: contacting the sample with a nucleic acid molecule which acts as a substrate for nucleic acid modifying activity of the micro-organism in the sample, incubating the thus contacted sample under conditions suitable for nucleic acid modifying activity; and specifically determining the absence or presence of a modified nucleic acid molecule resulting from the action of the nucleic acid modifying activity on the substrate nucleic acid molecule to indicate the absence or presence of the micro-organism. Corresponding kits are also provided.
Type:
Grant
Filed:
July 10, 2015
Date of Patent:
October 6, 2020
Assignee:
MOMENTUM BIOSCIENCE LIMITED
Inventors:
Matthew A. Crow, Helen V. Bennett, Daniel S. Wratting, William H. Mullen
Abstract: A method of detecting a ligase expressing micro-organism in a sample comprises steps of treating the sample under conditions that inhibit the activity of ATP-dependent ligase from mammalian cells but which do not inhibit the activity of the microbial ligases, contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for ligase activity in the sample, incubating the thus contacted sample under conditions suitable for ligase activity; and specifically determining the presence and/or the amount of a ligated nucleic acid molecule resulting from the action of the ligase on the substrate nucleic acid molecule to indicate the presence of the ligase expressing micro-organism. The micro-organism may be a fungus or a bacterium or both. High pH conditions may be employed to inactivate mammalian ligases. Related kits are described.
Abstract: Methods of detecting the absence or presence of a micro-organism in a sample comprising: contacting the sample with a nucleic acid molecule which acts as a substrate for nucleic acid modifying activity of the micro-organism in the sample, incubating the thus contacted sample under conditions suitable for nucleic acid modifying activity; and specifically determining the absence or presence of a modified nucleic acid molecule resulting from the action of the nucleic acid modifying activity on the substrate nucleic acid molecule to indicate the absence or presence of the micro-organism. Corresponding kits are also provided.
Type:
Application
Filed:
July 10, 2015
Publication date:
August 24, 2017
Applicant:
Momentum Bioscience Limited
Inventors:
Matthew A. Crow, Helen V. Bennett, Daniel S. Wratting, William H. Mullen