Abstract: A protein which satisfies all the biological criteria which are characteristic of inhibin has been isolated from a gonadal source. The purification and characterization of inhibin and the use of the purified material to raise antibodies, the use of inhibin and said antisera in a quantative radioimmunoassay, and application in vitro and in vivo of inhibin and antibody to inhibin, are described. There is provided a purified protein, inhibin characterized in that the apparent molecular weight as determined by SDS-PAGE is 56,000.+-.1,000, the isoelectric point is in the range 6.9-7.3, and the protein can bind specifically to Concanavalin A-Sepharose. Moreover, the protein includes two subunits, characterized in that their apparent molecular weights as determined by SDS-PAGE are 44,000.+-.3,000 and 14,000.+-.2,000, respectively. Furthermore, the isoelectric point of the 44,000 molecular weight sub-unit is in the range of 6.0-7.0. In addition, the N-terminal amino acid sequence of the two subunits are described.
Type:
Grant
Filed:
August 3, 1989
Date of Patent:
November 15, 1994
Assignees:
Biotechnology Australia Pty. Ltd, Monash University, St. Vincent's Institute of Medical Research, Monash Medical Center
Inventors:
Henry G. Burger, David M. de Kretser, John K. Findlay, Francis J. Morgan, Milton T. W. Hearn, David M. Milne-Robertson, Robert G. Forage