Abstract: A method for modifying a foreign nucleotide sequence for enhanced accumulation of its protein product in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by reducing the frequency of the rare and semi-rare monocotyledonous codons in the foreign gene and replacing them with more preferred monocotyledonous codons is disclosed. In addition, a method for enhancing the accumulation of a polypeptide encoded by a nucleotide sequence in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by analyzing the coding sequence in successive six nucleotide fragments and altering the sequence based on the frequency of appearance of the six-mers as to the frequency of appearance of the rarest 284, 484, and 664 six-mers in monocotyledonous plants is provided.
Type:
Grant
Filed:
August 5, 1997
Date of Patent:
January 30, 2001
Assignee:
Monsato Company
Inventors:
Sherri Marie Brown, Duff Allen Dean, Michael Ernest Fromm, Patricia Rigden Sanders
Abstract: A process and apparatus for removal of volatile divalent sulfur compounds from waste gases. Volatile organic compounds are advantageously removed in the same process. The divalent sulfur compounds are oxidized in a combustion zone, then preferably removed from the combustion gas, as by absorption or adsorption. The divalent sulfur compound and any VOCs are oxidized in either a catalytic or thermal combustion zone, after which the combustion gases are cooled by transfer of heat to the contaminated gases entering the combustion zone. A regenerative heat transfer reaction system is preferably used. Catalyst may comprise a noble metal, or oxides of Cr, Cu and/or Mn. A catalyst degraded by deposition of sulfate or sulfite salts on its surface may be reactivated by in situ decomposition of such salts. Reactivation also removes such salts from the heat transfer storage beds of a regenerative heat transfer reaction system.
Abstract: The invention relates to a method of improving the quality of potatoes stored at reduced temperatures and a method of prolonging dormancy of stored potato tubers, by increasing the level of ADPglucose pyrophosphorylase enzyme activity within the potato tuber during storage at ambient or reduced temperatures. Novel DNA molecules, plant cells, and potato plants are provided which contain the gene for the ADPglucose pyrophosphorylase enzyme.
Type:
Grant
Filed:
March 20, 1995
Date of Patent:
July 15, 1997
Assignee:
Monsato Company
Inventors:
Gerard Francis Barry, Ganesh Murthy Kishore, David Martin Stark, James Conrad Zalewski
Abstract: A process for preparing a controlled functional density poly(secondary amine) comprising: (a) contacting a polymer containing olefinic unsaturation with carbon monoxide in hydrogen under hydroformylation conditions in the presence of a hydroformylation catalyst to produce a controlled functional density polyaldehyde containing reactive carbon-carbon double bonds, and (b) contacting the polyaldehyde with hydrogen and a primary amine under reductive amination conditions in the presence of a ruthenium-containing imine hydrogenation catalyst or (b.sup.1) contacting the polyaldehyde with a sterically hindered aliphatic or cycloaliphatic primary amine or an aromatic primary amine under reductive amination conditions in the presence of an alkali metal borohydride to produce poly(secondary amine) having a substantially equivalent ratio of secondary amine groups to reactive carbon-carbon double bonds as the ratio of aldehyde groups to reactive carbon-carbon double bonds in the polyaldehyde.