Abstract: A pressure vessel balanced refuelling system enables greater volumes of gas to be used as working volumes and increases gas storage efficiency. The system includes a plurality of pressure vessels, each vessel having a liquid transfer opening for the entry and exit of a liquid that is used to displace a gas inside each vessel. A liquid transfer line extends from an outside to an inside of each vessel in the plurality of pressure vessels through the liquid transfer opening, and a liquid balance line inter-connects the liquid transfer opening of each vessel with the liquid transfer opening of each other vessel. Thus a balance liquid transfer path extends from an inside to an outside of one vessel, then from the outside to the inside of each other vessel through the liquid balance line, enabling a liquid level in the one vessel to remain approximately equal to a liquid level in each other vessel.

Abstract: A pressure vessel refueling system enables consistent mass flow rates and reduces the in-tank temperature rise caused by the heat of compression as gas is added to a vessel. The system includes a pressure vessel having a first gas inlet/outlet port and an interior cavity, and a nozzle is in fluid communication with the first gas inlet/outlet port. The nozzle and the pressure vessel are thermally coupled such that Joule-Thomson expansion of a gas flowing through the nozzle cools the interior cavity and contents of the pressure vessel.

Abstract: An improved vehicle fuel system enables mixing of natural gas and a liquid fuel upstream of a combustion cylinder. According to some embodiments the system includes: a gas pressure vessel and associated gas pressurization system to deliver natural gas at a desired pressure; a liquid fuel storage vessel and associated reservoir pump to deliver liquid fuel at a desired pressure; a mixing system configured to receive and mix the liquid fuel from the liquid fuel storage vessel and natural gas from the gas pressure vessel to produce a homogeneous fluid fuel mixture; and a common rail system connecting the mixing system to an engine that consumes the homogeneous fluid fuel.

Abstract: A pressure vessel refueling system enables fast refueling of Compressed Natural Gas (CNG) fuel tanks. The system includes a pressure vessel having a gas inlet/outlet port and a liquid inlet/outlet port; a first liquid at least partially filling the pressure vessel; a liquid layer of a second liquid floating on top of the first liquid, wherein the second liquid is immiscible with the first liquid; a gas at least partially filling the pressure vessel above the liquid layer of the second liquid, the gas in fluid communication with the gas inlet/outlet; and a pump in fluid communication with the liquid inlet/outlet of the pressure vessel, whereby the first liquid can be pumped or returned to/from storage into or out of the pressure vessel.

Abstract: A pressure vessel refuelling system enables consistent mass flow rates and reduces the in-tank temperature rise caused by the heat of compression as gas is added to a vessel. The system includes a pressure vessel having a first gas inlet/outlet port and an interior cavity, and a nozzle is in fluid communication with the first gas inlet/outlet port. The nozzle and the pressure vessel are thermally coupled such that Joule-Thomson expansion of a gas flowing through the nozzle cools the interior cavity and contents of the pressure vessel.

Abstract: A compressed gas transfer system comprising at least one first pressure vessel able to hold a volume of gas; and a first gas line to allow gas to pass out of the at least one first pressure vessel wherein the volume of the first pressure vessel is able to be varied to maintain the gas within the pressure vessel at a constant pressure.

Abstract: A compressed gas transfer system comprising at least one first pressure vessel able to hold a volume of gas; and a first gas line to allow gas to pass out of the at least one first pressure vessel wherein the volume of the first pressure vessel is able to be varied to maintain the gas within the pressure vessel at a constant pressure.

Abstract: A telecommunications system provides service to a cellular device located within a radio-frequency (RF) shadow of a communication station. The system may include a line-of-sight (LOS) antenna located in a line of sight of the station and a shadow antenna in communication with the LOS antenna and located within a line of sight of the RF shadow. The LOS antenna receives a transmitter signal from the station, and the shadow antenna receives a cellular signal from the cellular device. The LOS antenna also receives the cellular signal from the shadow antenna and, in turn, transmits the cellular signal to the station. Similarly, the shadow antenna receives the transmitter signal from the LOS antenna and, in turn, transmits the transmitter signal to the RF shadow. Accordingly, the cellular device is able to receive the transmitter signal T, and the station is able to receive the cellular signal.

Abstract: A universal gel and methods of use in detecting the presence, or absence, of one or more target molecules in a test sample comprising the formation of a universal gel hybridization complex, wherein an adapter molecule is hybridized to a target molecule and universal capture probe is disclosed.

Abstract: This invention features methods and apparatus for performing nucleic acid hybridization and amplification processes on a support. Such methods and apparatus are useful for synthesizing nucleic acid and detecting target nucleic acid for diagnostics and therapeutics.

Abstract: A multi-stage bridge amplification method which uses a recovered single-stranded amplification single-stranded nucleic acid molecule to initiate second and subsequent stages of bridge amplification is described.

Abstract: This invention features methods, apparatus and kits for performing nucleic acid hybridization and amplification reactions on a support. Such methods and apparatus are useful in diagnostic and therapeutic processes for synthesizing nucleic acid and detecting target nucleic acids in a sample.

Abstract: A method and apparatus for deciding whether to make a loan using an intangible asset, such as intellectual property, as collateral and for making such a loan more attractive to a lender. The method requires that an assessment of the transferability and viability of the asset be made to determine if the asset and loan applicant meet minimum qualifying criteria. If they do, a more detailed analysis is undertaken in which judgments are reached concerning various factors related to historical, comparative and prospective market behavior in market sectors identical with, as well as parallel and corollary to the primary market sector for the asset sought to be used as loan collateral. The analysis leads to calculation of an asset liquidation value and production of a correlated depreciation schedule which are both presented to the prospective lender.

Abstract: A multi-stage bridge amplification method which uses a recovered single-stranded amplification single-stranded nucleic acid molecule to initiate second and subsequent stages of bridge amplification is described.

Abstract: Methods for determining the presence of a target polynucleotide sequence using chemical hybridization in sequential probe and displacement complex formation with potential for signal gain prior to detection are disclosed.

Abstract: Devices and methods for detecting the presence, or absence of the presence of at least one target molecule employing a receptacle housing a reaction chamber comprised of at least one compartment containing suitable reagents for the detection of the target molecule are disclosed.

Abstract: The present invention pertains to a method and compositions for detecting the presence of a target nucleic acid in a test sample in which the sample to be tested for the presence of the target nucleic acid is introduced into a solution containing the probe-tether complex under conditions suitable for hybridization to occur between the first probe nucleic acid sequence and the target nucleic acid sequence so that the second tether nucleic acid sequence is displaced from the probe-tether complex, and a new complex is formed by the hybridization of the first probe nucleic acid sequence and the target nucleic acid sequence

Abstract: Methods are described for separation and analysis, and a kit for separation and analysis, of single nucleotide polymorphisms, or mutations, in target nucleic acid using denaturing gradient electrophoresis through supporting media containing one or more immobilized nucleic acid capture ligand. The method is especially useful for analyzing genetic haplotypes in samples with multiple linked polymorphic sites.

Abstract: Disclosed is a polymerizable complex containing a covalently attached nucleic acid molecule which, under appropriate conditions, is capable of copolymerization with a second polymerizable ethylene-containing monomer unit to form a polymerized layer. The polymerized layer containing attached nucleic acid is useful in a variety of contexts including, for example, hybridization assays. The polymerized layer containing the covalently attached nucleic acid molecule can be formed into a variety of shapes, or attached to a formed material through appropriate chemistry.

Abstract: A method has been developed which utilizes functionalized optical fibers as a solid support for the assembly of combinatorial libraries of compounds. The optical fibers are used to direct light, heat or a combination thereof to the compounds tethered to the fibers surface. Utilizing the method of the instant invention reactions occurring by both photochemically and thermally allowed pathways are accessible. In a preferred embodiment of the invention, the optical fibers are used to screen the array by fluorescence spectroscopy of labeled target molecules which bind to the immobilized library compounds.