Abstract: A valve assembly kit consisting of a plurality of specialized surgical instruments used for a reproducible, standardized technique to rapidly assemble a heart valve in the operating room from autologous tissue. The specialized surgical instruments of the valve assembly kit of the present invention are used to tailor, process (preserve) and assemble an autologous pericardium heart valve in relation with the anatomical configuration of the left ventricle and mitral annulus. The valve assembly kit generally consists of a tissue processing tray that supports the following specialized surgical instruments/devices: a tissue cutting device, an obturator with a removable handle, a valve assembly tray, and a universal stentless valve holder. These specialized surgical instruments will be used to rapidly (15-20 min.) assemble a heart valve in the operating room, at the time of the mitral valve replacement, from autologous tissue.

Abstract: A method for the rapid chemical treatment of autologous connective tissues which enhances biocompatibility by reducing cytotoxicity, immunogenicity and calcification without impairing the mechanical properties of the fixed tissue. The procedure includes a short exposure of the autologous biological tissue to a dialdehyde fixative followed by the rapid neutralization of excess aldehyde with an aminoacid solution.

Abstract: A cardiac valve comprises a plurality of flexible trapezoidal membranes, each having an edge joined to an edge of another of the membranes to form an unsupported frusto-conical body. The body has an oval end portion and a plurality of flexible flap portions integral with and extending from the end portions, the flap portions being formed by substantially parabolic scallops. The membranes are so sized, and the scallops are so positioned as to form flap portions of unequal size. The flexible membranes may be provided to the surgeon as separate membranes for construction of the artificial valve, or the valve may be provided to the surgeon as a complete unit. Alternatively, the surgeon may construct a valve using pericardium mapped and sized in accordance with the present invention.