Abstract: The present invention relates to a method for manufacturing mycelium mat into leather by using pH-controlled tannic acid. The method comprises the following steps: (1) inactivating the mycelium mat; (2) infiltrating the mycelium mat with a polysaccharide solution; (3) treating the polysaccharide-infused mycelium mat with a tannic acid solution; and (4) introducing a buffering agent to the tannic acid-treated mycelium mat. This innovative approach enhances the mechanical properties of the resulting leather, offering an environmentally friendly alternative to conventional leather materials.