Abstract: The present disclosure relates to methods for chitosan production. In particular, the present disclosure relates to a method for chitosan production comprising growing a biomass comprising an organic acid-producing, chitosan-containing fungus in an aqueous medium. A chitosan-reduced biomass and a chitosan-enriched aqueous medium are obtained. The chitosan-reduced biomass is separated from the chitosan-enriched aqueous medium. The chitosan is then separated from the chitosan-enriched aqueous medium, for example by adding a base to the chitosan-enriched aqueous medium under conditions to precipitate the chitosan and separating the precipitated chitosan from the aqueous medium.