Abstract: The present invention relates to a method for producing a cardiomyocyte. In more detail, the present invention relates to a method for producing a cell population containing cardiomyocytes, including differentiation process from cells capable of differentiating into cardiomyocytes to cardiomyocytes, and the differentiation process includes culturing the cells in a serum-free medium containing ? fetoprotein to obtain the cardiomyocytes.
Type:
Application
Filed:
January 12, 2024
Publication date:
September 19, 2024
Applicants:
MITSUBISHI CHEMICAL CORPORATION, MYORIDGE CO. LTD.
Abstract: A method for testing drug response of a cardiomyocyte, the method comprising: testing a response of the cardiomyocyte to an added drug in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less; or testing a response of the cardiomyocyte to an added drug immediately after placing the cardiomyocyte in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less.
Abstract: A method for testing drug response of a cardiomyocyte, the method comprising: testing a response of the cardiomyocyte to an added drug in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less; or testing a response of the cardiomyocyte to an added drug immediately after placing the cardiomyocyte in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocyte is 5.0 mm or less.
Abstract: A method for producing a target cell includes producing a target cell by culturing a raw material cell in a serum-free medium containing: at least one culture factor selected from a growth factor and a differentiation inducing factor; human serum albumin; and at least one additive selected from a group consisting of a Src inhibitor, a PKC activator, methylcellulose, an Essential 8 medium, recombinant human serum albumin, and non-human animal serum albumin.
Type:
Application
Filed:
May 17, 2021
Publication date:
August 3, 2023
Applicant:
MYORIDGE CO. LTD.
Inventors:
Itsunari Minami, Itsuro Motegi, Koji Eto
Abstract: A method for producing a cell sheet, the method including subjecting animal cells dispersed in a culture medium to a suspension culture while gathering the animal cells toward the center of the culture medium, to form a cell sheet floating in the culture medium.