Patents Assigned to NAN-NING INSTITUTE OF TRANSFUSION MEDICINE
  • Patent number: 10711046
    Abstract: A method for establishing eukaryotic expression cell line of CD36 mutant gene that encodes CD36 deficiency, the method including: (1) extracting total RNA from a whole blood sample derived from a CD36-deficient individual, and amplifying a coding sequence (CDS) in CD36 mRNA, to obtain a cDNA sequence fragment of the mutant CD36 gene; (2) splicing and amplifying the mutant CD36 gene and the EGFP fluorescent gene by SOE-PCR (Gene Splicing By Overlap Extension PCR) using four forward and reverse primers, to obtain a mutant gene fragment of MT-CD36-EGFP; (3) constructing and amplifying a MT-CD36-EGFP-pLV4/StripII-HIS10 eukaryotic expression vector including the mutant CD36 gene and the EGFP fluorescent gene; (4) transfecting the MT-CD36-EGFP-pLV4/StripII-HIS10 eukaryotic expression vector into the CHO-K1 cell line by using virus-mediated transfection of eukaryotic cells.
    Type: Grant
    Filed: November 12, 2018
    Date of Patent: July 14, 2020
    Assignee: NAN-NING INSTITUTE OF TRANSFUSION MEDICINE
    Inventors: Guoguang Wu, Lilan Li, Lihong Jiang, Haiyan Li, Jierun Chen