Abstract: The present disclosure relates to a field of compounds, and more particularly, a stable composition including angiotensin II, a preparation method, and an application method thereof. The composition may include: the angiotensin II; and an excipient, including one or more substances selected from a sugar, an alcohol, a polymer, a surfactant, or an amino acid. The present disclosure may be used for the stable composition including the angiotensin II with high stability, and may be stored under room temperature for more than two years, which solves a problem that the poor stability of the angiotensin II requires cold chain transportation and storage, thereby greatly improving the security and effectiveness of the medicine.