Abstract: Systems, devices, and methods for injecting biological material into a micro-object such as a cell are provided. In one aspect, for example, a cellular injection device can include a housing, an injection lance coupled to the housing and having a working tip extending outward from the housing, and a biological material delivery device coupled to the housing and having an effluent tip extending outward from the housing. The effluent tip can be positioned sufficiently proximal to the working tip such that biological material expelled from the effluent tip substantially contacts the working tip. In one aspect, the injection lance is removably coupled to the housing. In another aspect, the biological material delivery device is removably coupled to the housing.

Abstract: Systems, devices, and methods for restraining a micro-object are provided. In one aspect, a micro-object restraint device is provided having a micro-barrier structure coupled to a support substrate, where the micro-barrier structure has two micro-object holding regions. Each micro-object holding region includes a micro-object receiving opening defined in the micro-barrier structure, a micro-object impeding opening at an internal region of the micro-barrier structure, and at least two contact points positioned adjacent to the micro-object impeding opening and oriented to contact and impede a micro-object at the micro-object opening. The two micro-object holding regions abut one another and the micro-object openings from the two micro-object holding regions are continuous.

Abstract: Systems, devices, and methods for delivering a biological material into a biological structure are provided. In one aspect, for example, a lance device for introducing biological material into a biological structure and configured for use in a nanoinjection system including a microscope is provided. Such a device can include a substrate including a handle region located between a manipulator coupling region and a lance shaft region, a lance tip operable to introduce biological material into a biological structure, the lance tip being coupled to the lance shaft region, and an electrically conductive layer extending from the manipulator coupling region to the lance tip, the conductive layer being configured to electrically couple to a power source. Thus, the conductive layer provides an electrical connection from the power source to the lance tip when in use.