Abstract: Systems, devices, and methods of detecting nucleic acids may include a nanopore system or use of a nanopore system. The method of detecting a target nucleic acid may include combining a sample with at least one with at least one probe molecule having a sequence fully complementary or partially complementary to the target nucleic acid, such that the probe molecule hybridizes to the target nucleic acid, and adding one of more enzymes before or after combining the sample with the probe molecule(s). The sample may be added to a chamber of a nanopore system and a voltage applied to generate a current time series, wherein a signature current pattern of the nanopore system indicates the presence of the target nucleic acid in the sample.