Abstract: A microstructured measurement chip (1) for optical measurement of properties of artificial or biological membranes (40), having a lower, translucent support layer (10) and at least one non-translucent main layer (20) disposed on top of the former, which layer has depressions (30) configured as measurement chambers, having an upper opening (25) and one or multiple inner side walls (26). In order to improve the measurement chip (1) in such a manner that biological systems can be measured with greater measurement accuracy and higher throughput, it is proposed that the side wall or the side walls (26) of the measurement chambers (30) have depressions (27) and/or elevations (28). The invention furthermore relates to a holder (200) for the measurement chips (1) as well as to a method for the production of the measurement chips (1) from a silicon wafer (300).