Abstract: A microfluidic apparatus and methods thereof. The Apparatus having a flat and thin substrate, the substrate including at least one microfluidic testing device, each device with: plurality of Stationary Nanoliter Droplet Array (SNDA) components; a common inlet port and a distribution manifold, configured to enable an introduction of a fluid into all the primary channels; plurality of individual inlet ports, each coupled to a different primary channel, configured to enable an individual introduction of a fluid into its associated primary channel; and one or more outlet ports and optionally a collecting manifold, configured to evacuate liquid and/or gas flowing out thereof.

Abstract: Microfluidic devices and methods thereof; the devices including: SNDA components; each SNDA component comprising: a primary channel; secondary channels; and nano-wells that are each open to the primary channel and are each connected via vents to the secondary channel; the vents are configured to enable passage of gas solely from the nano-wells to the secondary channel, such that when a fluid is introduced into the primary channel it fills the nano-wells, and the originally accommodated gas is evacuated via the vents and the secondary channel/s; a common inlet port, configured to enable a simultaneous introduction of the fluid into all the primary channels of the different SNDA components; individual inlet ports, configured to enable individual introduction of fluid, each into a different primary channel of a different SNDA component; and at least one outlet port, configured to enable evacuation of the gas out of all the secondary channels.