Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Application
Filed:
January 30, 2020
Publication date:
May 21, 2020
Applicant:
Natera, Inc.
Inventors:
Joshua BABIARZ, Tudor Pompiliu CONSTANTIN, Lane A. EUBANK, George GEMELOS, Matthew Micah HILL, Huseyin Eser KIRKIZLAR, Matthew RABINOWITZ, Onur SAKARYA, Styrmir SIGURJONSSON, Bernhard ZIMMERMANN
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Grant
Filed:
March 14, 2019
Date of Patent:
May 19, 2020
Assignee:
Natera, Inc.
Inventors:
Joshua Babiarz, Tudor Pompiliu Constantin, Lane A. Eubank, George Gemelos, Matthew Micah Hill, Huseyin Eser Kirkizlar, Matthew Rabinowitz, Onur Sakarya, Styrmir Sigurjonsson, Bernhard Zimmermann
Abstract: A system and method for determining the genetic data for one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available, are disclosed. Genetic data for the target individual is acquired and amplified using known methods, and poorly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related subjects. In accordance with one embodiment of the invention, incomplete genetic data is acquired from embryonic cells, fetal cells, or cell-free fetal DNA isolated from the mother's blood, and the incomplete genetic data is reconstructed using the more complete genetic data from a larger sample diploid cells from one or both parents, with or without genetic data from haploid cells from one or both parents, and/or genetic data taken from other related individuals.
Type:
Grant
Filed:
April 30, 2019
Date of Patent:
May 19, 2020
Assignee:
Natera, Inc.
Inventors:
Matthew Rabinowitz, Milena Banjevic, Zachary Demko, David Johnson
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Application
Filed:
January 21, 2020
Publication date:
May 14, 2020
Applicant:
Natera, Inc.
Inventors:
Joshua BABIARZ, Tudor Pompiliu CONSTANTIN, Lane A. EUBANK, George GEMELOS, Matthew Micah HILL, Huseyin Eser KIRKIZLAR, Matthew RABINOWITZ, Onur SAKARYA, Styrmir SIGURJONSSON, Bernhard ZIMMERMANN
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Application
Filed:
January 15, 2020
Publication date:
May 7, 2020
Applicant:
Natera, Inc.
Inventors:
Joshua BABIARZ, Tudor Pompiliu CONSTANTIN, Lane A. EUBANK, George GEMELOS, Matthew Micah HILL, Huseyin Eser KIRKIZLAR, Matthew RABINOWITZ, Onur SAKARYA, Styrmir SIGURJONSSON, Bernhard ZIMMERMANN
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Application
Filed:
January 6, 2020
Publication date:
April 23, 2020
Applicant:
Natera, Inc.
Inventors:
Joshua BABIARZ, Tudor Pompiliu CONSTANTIN, Lane A. EUBANK, George GEMELOS, Matthew Micah HILL, Huseyin Eser KIRKIZLAR, Matthew RABINOWITZ, Onur SAKARYA, Styrmir SIGURJONSSON, Bernhard ZIMMERMANN
Abstract: Provided herein are improved methods for detecting aneuploidy in a sample. The methods in certain embodiments are used for the analysis of circulating DNA in serum samples, such as circulating fetal DNA or circulating tumor DNA. In certain embodiments, chromosome or chromosome segments of interest are used to set a bias model and/or a control value for a z- score determination, in illustrative examples without the use of a control chromosome.
Abstract: A system and method for determining the genetic data for one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available, are disclosed. Genetic data for the target individual is acquired and amplified using known methods, and poorly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related subjects. In accordance with one embodiment of the invention, incomplete genetic data is acquired from embryonic cells, fetal cells, or cell-free fetal DNA isolated from the mother's blood, and the incomplete genetic data is reconstructed using the more complete genetic data from a larger sample diploid cells from one or both parents, with or without genetic data from haploid cells from one or both parents, and/or genetic data taken from other related individuals.
Type:
Grant
Filed:
May 14, 2019
Date of Patent:
March 24, 2020
Assignee:
Natera, Inc.
Inventors:
Matthew Rabinowitz, Milena Banjevic, Zachary Demko, David Johnson
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Grant
Filed:
April 30, 2019
Date of Patent:
March 24, 2020
Assignee:
Natera, Inc.
Inventors:
Bernhard Zimmermann, Matthew Hill, Philippe Lacroute, Michael Dodd, Alexander Wong
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Grant
Filed:
May 14, 2019
Date of Patent:
March 24, 2020
Assignee:
Natera, Inc.
Inventors:
Bernhard Zimmermann, Matthew Hill, Philippe Lacroute, Michael Dodd, Alexander Wong
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Grant
Filed:
February 27, 2019
Date of Patent:
March 24, 2020
Assignee:
Natera, Inc.
Inventors:
Joshua Babiarz, Tudor Pompiliu Constantin, Lane A. Eubank, George Gemelos, Matthew Micah Hill, Huseyin Eser Kirkizlar, Matthew Rabinowitz, Onur Sakarya, Styrmir Sigurjonsson, Bernhard Zimmermann
Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.
Type:
Grant
Filed:
April 30, 2019
Date of Patent:
March 17, 2020
Assignee:
Natera, Inc.
Inventors:
Allison Ryan, Styrmir Sigurjonsson, Milena Banjevic, George Gemelos, Matthew Hill, Johan Baner, Matthew Rabinowitz, Zachary Demko
Abstract: The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.
Type:
Grant
Filed:
September 26, 2017
Date of Patent:
March 3, 2020
Assignee:
Natera, Inc.
Inventors:
Bernhard Zimmermann, Ryan Swenerton, Matthew Rabinowitz, Styrmir Sigurjonsson, George Gemelos, Apratim Ganguly, Himanshu Sethi
Abstract: Embodiments of the invention include methods and compositions for producing standards for noninvasive prenatal genetic diagnostics and for the detection and monitoring of cancer. The compositions can include a plurality of different nucleosomal DNA fragments derived from either primary cells or cell lines and can include one or more synthetic oligonucleotides. The amount of the different nucleosomal DNA fragments can be varied so as to simulate naturally occurring cell free DNA samples obtained from the blood of the pregnant woman or naturally occurring cell free DNA samples obtained from the blood of cancer patients.
Type:
Grant
Filed:
January 14, 2016
Date of Patent:
March 3, 2020
Assignee:
Natera, Inc.
Inventors:
Joshua Babiarz, Bernhard Zimmermann, Tudor Pompiliu Constantin
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Grant
Filed:
April 30, 2019
Date of Patent:
February 11, 2020
Assignee:
Natera, Inc.
Inventors:
Joshua Babiarz, Tudor Pompiliu Constantin, Lane A. Eubank, George Gemelos, Matthew Micah Hill, Huseyin Eser Kirkizlar, Matthew Rabinowitz, Onur Sakarya, Styrmir Sigurjonsson, Bernhard Zimmermann
Abstract: Disclosed here is a method for detecting genome rearrangement in a biological sample, comprising: obtaining a contact matrix plotted from proximity ligation sequencing data of at least one chromosome; identifying an abnormal contact pattern in the contact matrix compared to the contact matrix of a reference genome; comparing the abnormal contact pattern in the contact matrix to one or more known patterns associated with genomic rearrangement to identify a type of genomic rearrangement causing the abnormal contact pattern.
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Grant
Filed:
April 30, 2019
Date of Patent:
January 21, 2020
Assignee:
Natera, Inc.
Inventors:
Joshua Babiarz, Tudor Pompiliu Constantin, Lane A. Eubank, George Gemelos, Matthew Micah Hill, Huseyin Eser Kirkizlar, Matthew Rabinowitz, Onur Sakarya, Styrmir Sigurjonsson, Bernhard Zimmermann
Abstract: The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.
Type:
Grant
Filed:
September 26, 2017
Date of Patent:
January 14, 2020
Assignee:
Natera, Inc.
Inventors:
Bernhard Zimmermann, Ryan Swenerton, Matthew Rabinowitz, Styrmir Sigurjonsson, George Gemelos, Apratim Ganguly, Himanshu Sethi
Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
Type:
Grant
Filed:
May 14, 2019
Date of Patent:
January 7, 2020
Assignee:
Natera, Inc.
Inventors:
Joshua Babiarz, Tudor Pompiliu Constantin, Lane A. Eubank, George Gemelos, Matthew Micah Hill, Huseyin Eser Kirkizlar, Matthew Rabinowitz, Onur Sakarya, Styrmir Sigurjonsson, Bernhard Zimmermann
Abstract: Disclosed herein are methods for determining the copy number of a chromosome in a fetus in the context of non-invasive prenatal diagnosis. In an embodiment, the measured genetic data from a sample of genetic material that contains both fetal DNA and maternal DNA is analyzed, along with the genetic data from the biological parents of the fetus, and the copy number of the chromosome of interest is determined. In an embodiment, the maternal serum is measured using a single-nucleotide polymorphism (SNP) microarray, along with parental genomic data, and the determination of the chromosome copy number is used to make clinical decisions pertaining to the fetus.
Type:
Grant
Filed:
November 3, 2016
Date of Patent:
December 31, 2019
Assignee:
Natera, Inc.
Inventors:
Matthew Rabinowitz, Allison Ryan, George Gemelos, Milena Banjevic, Zachary Demko