Abstract: The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed accumulation of degrading bacteria, which contains a porous material provided as a support on which a degrading bacterium A capable of degrading at least one organic contaminant and a degrading bacterium B capable of degrading another organic contaminant are accumulated, is produced. The degrading bacterium A may be a PCNB-degrading bacterium, particularly a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics of Nocardioides sp. PD653 and the degrading bacterium B may be a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics of ?-Proteobacteria CDB21.
Type:
Grant
Filed:
September 16, 2009
Date of Patent:
October 9, 2012
Assignees:
National Institute for Agro-Environmental Sciences Independent Administrative Institute, Kowa Co., Ltd.
Abstract: The object of the present invention is to restore soil and prevent contamination of ground water by decomposing an organic compound with decomposing microorganisms in soil contaminated with an agricultural chemical or other organic compound.
Type:
Grant
Filed:
April 20, 2001
Date of Patent:
May 27, 2003
Assignees:
National Institute for Agro-Environmental Sciences
Independent Administrative Institute, Toyo Denka Kogyo Co., Ltd.
Abstract: It is an object of the invention to improve a conventional soil percolation technique to thereby provide a method of enriching and isolating decomposing bacteria decomposing an organochlorine agricultural chemical PCNB which is difficult to decompose, in a short time period, and to provide decomposing bacteria for efficiently processing PCNB. To practice the method, an enrichment soil layer 2 is formed by mixing a soil containing an organochlorine agricultural chemical PCNB with a fragmented porous material having an infinite number of micropores and at the same time a greater adsorptivity for adsorbing PCNB than the soil, and an inorganic salt medium 3 containing a carbon and nitrogen source, the carbon and nitrogen source being formed by only PCNB, is circulated through the enrichment soil layer 2, thereby enriching the aerobic bacteria Burkholderia cepacia in the fragmented porous material.
Type:
Grant
Filed:
March 24, 2000
Date of Patent:
December 24, 2002
Assignee:
National Institute for Agro-Environmental Science Independent
Administrative Institute
Abstract: A porous material having a greater adsorptivity for adsorbing organic contaminants, such as agricultural chemicals, than a target soil, is fragmented. The fragmented porous material is mixed into a soil which the decomposing bacteria inhabit, thereby forming an enrichment soil layer. Through the enrichment soil layer, there is circulated an inorganic salt medium containing carbon and nitrogen sources formed by only an organic contaminant to be decomposed, thereby rapidly enriching the decomposing bacteria in the fragmented porous material. The fragmented porous material is inoculated into new fragmented porous material to form an enrichment layer consisting of the fragmented porous material only.
Type:
Grant
Filed:
August 23, 1999
Date of Patent:
September 17, 2002
Assignee:
National Institute for Agro-Environmental Sciences
Independent Administrative Institute