Patents Assigned to National Institute for Environmental Studies
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Patent number: 11415563Abstract: In an LC/MS/MS analysis: injecting a sample into a passage leading to a column group provided in a liquid chromatograph, the column group including a plurality of columns serially connected to each other and packed with different kinds of packing materials; supplying an eluant to one or a plurality of columns including a column located most downstream, to separate a portion of the target components in the sample in the one or plurality of columns, and sequentially elute those components from the most downstream column to perform mass spectrometry; and supplying a different eluant to one or a plurality of columns including the most downstream column, to separate at least a portion of the target components which stayed uneluted in the one or plurality of columns in the first analysis step, and sequentially elute those components from the most downstream column to perform mass spectrometry.Type: GrantFiled: April 13, 2017Date of Patent: August 16, 2022Assignees: SHIMADZU CORPORATION, NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIESInventors: Toshikazu Minohata, Jun Watanabe, Shoji Nakayama
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Publication number: 20220163450Abstract: The present invention provides a device (1) for detecting contamination with a photosynthesis inhibitor, the device including a collection device (10) configured to collect a test liquid, a light-blocking pretreatment tank (21) configured to store the test liquid (2) collected by the collection device in a state of containing phytoplankton, a stirring device configured to maintain a floating state of the phytoplankton in the test liquid stored in the pretreatment tank, an irradiation light source configured to irradiate the phytoplankton in the test liquid stored in the pretreatment tank with weak light having an underwater photon flux density that does not cause photoinhibition, a drainage conduit (40) configured to allow the test liquid discharged from the pretreatment tank to flow thereinto, and a fluorescence quantum efficiency measuring machine (50) provided for the drainage conduit and configured to measure a fluorescence quantum efficiency of the phytoplankton in the test liquid discharged from the preType: ApplicationFiled: February 6, 2020Publication date: May 26, 2022Applicants: National Institute for Environmental Studies, Japan Agency for Marine-Earth Science and TechnologyInventors: Hiroshi Koshikawa, Masanobu Kawachi, Shigeshi Fuchida, Shuhei Ota, Tetsuya Miwa, Yasuo Furushima, Shunsuke Kondo
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Publication number: 20200158701Abstract: In an LC/MS/MS analysis: injecting a sample into a passage leading to a column group provided in a liquid chromatograph, the column group including a plurality of columns serially connected to each other and packed with different kinds of packing materials; supplying an eluant to one or a plurality of columns including a column located most downstream, to separate a portion of the target components in the sample in the one or plurality of columns, and sequentially elute those components from the most downstream column to perform mass spectrometry; and supplying a different eluant to one or a plurality of columns including the most downstream column, to separate at least a portion of the target components which stayed uneluted in the one or plurality of columns in the first analysis step, and sequentially elute those components from the most downstream column to perform mass spectrometry.Type: ApplicationFiled: April 13, 2017Publication date: May 21, 2020Applicants: SHIMADZU CORPORATION, National Institute for Environmental StudiesInventors: Toshikazu MINOHATA, Jun WATANABE, Shoji NAKAYAMA
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Patent number: 10643830Abstract: A sample introduction unit 2 regularly collects a sample from a sample passage 1. A measurement unit 3 performs an LC/MS analysis for the collected sample. A spectrum matching judgment section 42 performs image recognition referring to mass spectra in a normal condition accumulated in a normal spectrum database 44, to determine whether a measured mass spectrum is in a normal condition. If the mass spectrum is non-normal, an abnormal peak locator 45 locates a peak which should not be detected in the normal condition, and an MSn analysis with the located peak selected as the target is performed in the measurement unit 3 under an MSn analysis execution commander 46. An identification processor 47 performs substance identification by a library search based on an acquired MSn spectrum. Upon successful identification, an alert commander 49 issues an alert. Thus, the system can automatically monitor hazardous substances with high accuracy.Type: GrantFiled: October 12, 2018Date of Patent: May 5, 2020Assignees: SHIMADZU CORPORATION, National Institute for Environmental StudiesInventors: Toshikazu Minohata, Shoji Nakayama
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Patent number: 10463547Abstract: The purpose of the present disclosure is to provide a small-sized electric vehicle that is capable of assisting power of a wheelchair coupled to the small-sized electric vehicle, and that is capable of traveling alone in a state of being detached from the wheelchair. A folding vehicle 1 is capable of making a wheelchair 101 coupled by a coupling member 61 move in a self-traveling manner using a battery 14 and an in-wheel motor M etc. Furthermore, the folding vehicle 1 is capable of self-traveling by using the battery 14 and the in-wheel motor M etc. while in an independent state of not being coupled to the wheelchair 101.Type: GrantFiled: August 7, 2018Date of Patent: November 5, 2019Assignees: TOYODA IRON WORKS CO., LTD., NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES, ACCURATE SYSTEMS INC.Inventors: Mitsutoshi Kano, Yoshinori Kondo, Junichi Yasu
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Patent number: 10407329Abstract: A wastewater treatment system includes: an acid fermentation tank; a methane fermentation tank positioned under the acid fermentation tank, and configured to produce biogas, and an upper portion of the methane fermentation tank including an opening that communicates with the acid fermentation tank; and a gas type liquid partition valve provided at the opening, including a gas storage portion configured to store a predetermined amount of the biogas and discharge the biogas exceeding the predetermined amount to the acid fermentation tank, and configured to block the opening with the biogas stored in the gas storage portion.Type: GrantFiled: January 19, 2017Date of Patent: September 10, 2019Assignee: National Institute for Environmental StudiesInventors: Takashi Onodera, Kazuaki Syutsubo, Motoyuki Mizuochi
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Publication number: 20190115197Abstract: A sample introduction unit 2 regularly collects a sample from a sample passage 1. A measurement unit 3 performs an LC/MS analysis for the collected sample. A spectrum matching judgment section 42 performs image recognition referring to mass spectra in a normal condition accumulated in a normal spectrum database 44, to determine whether a measured mass spectrum is in a normal condition. If the mass spectrum is non-normal, an abnormal peak locator 45 locates a peak which should not be detected in the normal condition, and an MSn analysis with the located peak selected as the target is performed in the measurement unit 3 under an MSn analysis execution commander 46. An identification processor 47 performs substance identification by a library search based on an acquired MSn spectrum. Upon successful identification, an alert commander 49 issues an alert. Thus, the system can automatically monitor hazardous substances with high accuracy.Type: ApplicationFiled: October 12, 2018Publication date: April 18, 2019Applicants: SHIMADZU CORPORATION, NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIESInventors: Toshikazu MINOHATA, Shoji NAKAYAMA
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Publication number: 20190031542Abstract: A wastewater treatment system includes: an acid fermentation tank; a methane fermentation tank positioned under the acid fermentation tank, and configured to produce biogas, and an upper portion of the methane fermentation tank including an opening that communicates with the acid fermentation tank; and a gas type liquid partition valve provided at the opening, including a gas storage portion configured to store a predetermined amount of the biogas and discharge the biogas exceeding the predetermined amount to the acid fermentation tank, and configured to block the opening with the biogas stored in the gas storage portion.Type: ApplicationFiled: January 19, 2017Publication date: January 31, 2019Applicant: National Institute for Environmental StudiesInventors: Takashi ONODERA, Kazuaki SYUTSUBO, Motoyuki MIZUOCHI
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Publication number: 20180338877Abstract: The purpose of the present disclosure is to provide a small-sized electric vehicle that is capable of assisting power of a wheelchair coupled to the small-sized electric vehicle, and that is capable of traveling alone in a state of being detached from the wheelchair. A folding vehicle 1 is capable of making a wheelchair 101 coupled by a coupling member 61 move in a self-traveling manner using a battery 14 and an in-wheel motor M etc. Furthermore, the folding vehicle 1 is capable of self-traveling by using the battery 14 and the in-wheel motor M etc. while in an independent state of not being coupled to the wheelchair 101.Type: ApplicationFiled: August 7, 2018Publication date: November 29, 2018Applicants: TOYODA IRON WORKS CO., LTD., NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES, ACCURATE SYSTEMS INC.Inventors: Mitsutoshi KANO, Yoshinori KONDO, Junichi YASU
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Patent number: 8765473Abstract: A basement membrane having a barrier function is formed by culturing alveolar epithelial cells or vascular endothelial cells on a fibrous collagen matrix coated with a polymer having a sugar chain that can localize a receptor that has an activity to accumulate a basement membrane component on the basal surface of the cells having an ability to form a basement membrane. A reconstructed artificial tissue is obtained by seeding and culturing desired homogeneous or heterogeneous cells on the basement membrane specimen constructed by the following process: (i) the cells having an ability to form a basement membrane adhered onto a support structure through a basement membrane are treated with a surface active agent; (ii) the lipid component of cells is lysed; (iii) the mixture of an alkaline solution and a protease inhibitor is used to lyse the protein remained on the surface of the basement membrane of the cells.Type: GrantFiled: November 15, 2006Date of Patent: July 1, 2014Assignees: Japan Science and Technology Agency, National Institute for Environmental StudiesInventor: Katsumi Mochitate
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Patent number: 7972852Abstract: A reconstructed artificial tissue is obtained by seeding and culturing desired homogeneous or heterogeneous cells having an ability to form a basement membrane on the basement membrane specimen constructed by the following process: the cells having an ability to form a basement membrane adhered onto a support structure through a basement membrane are treated with a surface active agent; the lipid component of cells is lysed; the mixture of an alkaline solution and a protease inhibitor is used to lyse the protein remained on the surface of the basement membrane of the cells. A protein support structure is temporarily adhered to plastic surface through an adsorptive polymer by hydrophobic bonding, such as an alternating copolymer of methyl vinyl ether and maleic anhydride, which has a hydrophobic linear carbon skeleton and a functional group which can react with protein in a molecule and a basement membrane specimen or an artificial tissue is formed thereon.Type: GrantFiled: November 15, 2006Date of Patent: July 5, 2011Assignees: Japan Science and Technology Agency, National Institute for Environmental StudiesInventor: Katsumi Mochitate
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Patent number: 7525086Abstract: An in-spray glow discharge ionization method and apparatus are provided The in-spray glow discharge ionization apparatus has a supply port supplying a fluid containing a compound to be measured, a gas blowing port which surrounds the supply port and which blows a gas exhibiting Penning effect to nebulize the fluid supplied from the supply port, a ground-side discharge electrode provided at a generation port at which the nebulized flow is generated, and a voltage application-side discharge electrode which is disposed in the traveling direction of the nebulized flow and opposed to the ground-side discharge electrode. In this in-spray glow discharge ionization method, while the fluid is nebulized by a spray gas (1), components of the compound to be measured which constitutes the fluid are ionized by the excited spray gas (1) exhibiting Penning effect, so that measurement is performed by a mass spectrometer.Type: GrantFiled: November 21, 2003Date of Patent: April 28, 2009Assignees: Japan Science and Technology Agency, National Institute for Environmental StudiesInventor: Shigeru Suzuki
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Patent number: 7399634Abstract: The object of the present invention is to provide a method for preparing a basement membrane comprising extracellular matrices having a function to control morphology, differentiation, proliferation, motility, function expression and the like of cells. This basement membrane is formed by culturing cells having an ability to form a basement membrane on a fibrous collagen matrix coated with a polymer having a sugar chain. This sugar chain coat can localize a receptor which has an activity to accumulate a basement membrane component on the basal surface of the cells.Type: GrantFiled: March 25, 2004Date of Patent: July 15, 2008Assignees: Japan Science and Technology Agency, National Institute for Environmental StudiesInventor: Katsumi Mochitate
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Publication number: 20060263878Abstract: The present invention provides a cell culture substrate which effectively adsorbs to a cell culture substrate such as a well, which is excellent in cell adhesion reproducibility, and whose surface is coated with a hydrophobic binding-adsorptive polymer and an immobilized preparation of cell adhesion proteins or peptides which effectively binds to the cell culture substrate and which is excellent in cell adhesion reproducibility, and further, an artificial tissue prepared by seeding and culturing cells on the immobilized preparation of cell adhesion peptides.Type: ApplicationFiled: March 24, 2004Publication date: November 23, 2006Applicants: NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES, Katsumi MochitateInventor: Katsumi Mochitate
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Patent number: 6099731Abstract: A process for treating a water containing chlorinated organic compounds, wherein a microorganism is introduced from a degradation tank to a separation tank and an energy source is supplied to the microorganism separated in the separation tank. A water-treatment apparatus having a structure capable of continuously introducing water to be treated through a degradation tank, a separation tank and a reactivation tank. A loss of the energy source such as methane or propane discharged from the separation tank can be reduced. A water-treatment apparatus having a degradation tank and a separation tank, wherein a microorganism can be cultivated in the separation tank. The reactivation tank can be omitted, whereby the entire size of the apparatus can be reduced.Type: GrantFiled: September 11, 1998Date of Patent: August 8, 2000Assignees: Ebara Research Co., Ltd., Japan as represented by Director General of Agency of National Institute for Environmental StudiesInventors: Hiroo Uchiyama, Osami Yagi, Masayoshi Kitagawa, Tatsuo Shimomura, Takeshi Hasegawa
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Patent number: 5232855Abstract: The present invention provides an axenic mass culture apparatus comprising a light source for illuminating an entire surface of a culture liquid in a culture tank. According to the present invention, it is possible to achieve a light-illuminating type high-speed axenic mass culture of biotic cells.Type: GrantFiled: September 21, 1992Date of Patent: August 3, 1993Assignee: National Institute for Environmental StudiesInventors: Masataka Watanabe, Kunio Kohata