Patents Assigned to National Institute for Environmental Studies
  • Patent number: 11415563
    Abstract: In an LC/MS/MS analysis: injecting a sample into a passage leading to a column group provided in a liquid chromatograph, the column group including a plurality of columns serially connected to each other and packed with different kinds of packing materials; supplying an eluant to one or a plurality of columns including a column located most downstream, to separate a portion of the target components in the sample in the one or plurality of columns, and sequentially elute those components from the most downstream column to perform mass spectrometry; and supplying a different eluant to one or a plurality of columns including the most downstream column, to separate at least a portion of the target components which stayed uneluted in the one or plurality of columns in the first analysis step, and sequentially elute those components from the most downstream column to perform mass spectrometry.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: August 16, 2022
    Assignees: SHIMADZU CORPORATION, NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES
    Inventors: Toshikazu Minohata, Jun Watanabe, Shoji Nakayama
  • Publication number: 20220163450
    Abstract: The present invention provides a device (1) for detecting contamination with a photosynthesis inhibitor, the device including a collection device (10) configured to collect a test liquid, a light-blocking pretreatment tank (21) configured to store the test liquid (2) collected by the collection device in a state of containing phytoplankton, a stirring device configured to maintain a floating state of the phytoplankton in the test liquid stored in the pretreatment tank, an irradiation light source configured to irradiate the phytoplankton in the test liquid stored in the pretreatment tank with weak light having an underwater photon flux density that does not cause photoinhibition, a drainage conduit (40) configured to allow the test liquid discharged from the pretreatment tank to flow thereinto, and a fluorescence quantum efficiency measuring machine (50) provided for the drainage conduit and configured to measure a fluorescence quantum efficiency of the phytoplankton in the test liquid discharged from the pre
    Type: Application
    Filed: February 6, 2020
    Publication date: May 26, 2022
    Applicants: National Institute for Environmental Studies, Japan Agency for Marine-Earth Science and Technology
    Inventors: Hiroshi Koshikawa, Masanobu Kawachi, Shigeshi Fuchida, Shuhei Ota, Tetsuya Miwa, Yasuo Furushima, Shunsuke Kondo
  • Publication number: 20200158701
    Abstract: In an LC/MS/MS analysis: injecting a sample into a passage leading to a column group provided in a liquid chromatograph, the column group including a plurality of columns serially connected to each other and packed with different kinds of packing materials; supplying an eluant to one or a plurality of columns including a column located most downstream, to separate a portion of the target components in the sample in the one or plurality of columns, and sequentially elute those components from the most downstream column to perform mass spectrometry; and supplying a different eluant to one or a plurality of columns including the most downstream column, to separate at least a portion of the target components which stayed uneluted in the one or plurality of columns in the first analysis step, and sequentially elute those components from the most downstream column to perform mass spectrometry.
    Type: Application
    Filed: April 13, 2017
    Publication date: May 21, 2020
    Applicants: SHIMADZU CORPORATION, National Institute for Environmental Studies
    Inventors: Toshikazu MINOHATA, Jun WATANABE, Shoji NAKAYAMA
  • Patent number: 10643830
    Abstract: A sample introduction unit 2 regularly collects a sample from a sample passage 1. A measurement unit 3 performs an LC/MS analysis for the collected sample. A spectrum matching judgment section 42 performs image recognition referring to mass spectra in a normal condition accumulated in a normal spectrum database 44, to determine whether a measured mass spectrum is in a normal condition. If the mass spectrum is non-normal, an abnormal peak locator 45 locates a peak which should not be detected in the normal condition, and an MSn analysis with the located peak selected as the target is performed in the measurement unit 3 under an MSn analysis execution commander 46. An identification processor 47 performs substance identification by a library search based on an acquired MSn spectrum. Upon successful identification, an alert commander 49 issues an alert. Thus, the system can automatically monitor hazardous substances with high accuracy.
    Type: Grant
    Filed: October 12, 2018
    Date of Patent: May 5, 2020
    Assignees: SHIMADZU CORPORATION, National Institute for Environmental Studies
    Inventors: Toshikazu Minohata, Shoji Nakayama
  • Patent number: 10463547
    Abstract: The purpose of the present disclosure is to provide a small-sized electric vehicle that is capable of assisting power of a wheelchair coupled to the small-sized electric vehicle, and that is capable of traveling alone in a state of being detached from the wheelchair. A folding vehicle 1 is capable of making a wheelchair 101 coupled by a coupling member 61 move in a self-traveling manner using a battery 14 and an in-wheel motor M etc. Furthermore, the folding vehicle 1 is capable of self-traveling by using the battery 14 and the in-wheel motor M etc. while in an independent state of not being coupled to the wheelchair 101.
    Type: Grant
    Filed: August 7, 2018
    Date of Patent: November 5, 2019
    Assignees: TOYODA IRON WORKS CO., LTD., NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES, ACCURATE SYSTEMS INC.
    Inventors: Mitsutoshi Kano, Yoshinori Kondo, Junichi Yasu
  • Patent number: 10407329
    Abstract: A wastewater treatment system includes: an acid fermentation tank; a methane fermentation tank positioned under the acid fermentation tank, and configured to produce biogas, and an upper portion of the methane fermentation tank including an opening that communicates with the acid fermentation tank; and a gas type liquid partition valve provided at the opening, including a gas storage portion configured to store a predetermined amount of the biogas and discharge the biogas exceeding the predetermined amount to the acid fermentation tank, and configured to block the opening with the biogas stored in the gas storage portion.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: September 10, 2019
    Assignee: National Institute for Environmental Studies
    Inventors: Takashi Onodera, Kazuaki Syutsubo, Motoyuki Mizuochi
  • Publication number: 20190115197
    Abstract: A sample introduction unit 2 regularly collects a sample from a sample passage 1. A measurement unit 3 performs an LC/MS analysis for the collected sample. A spectrum matching judgment section 42 performs image recognition referring to mass spectra in a normal condition accumulated in a normal spectrum database 44, to determine whether a measured mass spectrum is in a normal condition. If the mass spectrum is non-normal, an abnormal peak locator 45 locates a peak which should not be detected in the normal condition, and an MSn analysis with the located peak selected as the target is performed in the measurement unit 3 under an MSn analysis execution commander 46. An identification processor 47 performs substance identification by a library search based on an acquired MSn spectrum. Upon successful identification, an alert commander 49 issues an alert. Thus, the system can automatically monitor hazardous substances with high accuracy.
    Type: Application
    Filed: October 12, 2018
    Publication date: April 18, 2019
    Applicants: SHIMADZU CORPORATION, NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES
    Inventors: Toshikazu MINOHATA, Shoji NAKAYAMA
  • Publication number: 20190031542
    Abstract: A wastewater treatment system includes: an acid fermentation tank; a methane fermentation tank positioned under the acid fermentation tank, and configured to produce biogas, and an upper portion of the methane fermentation tank including an opening that communicates with the acid fermentation tank; and a gas type liquid partition valve provided at the opening, including a gas storage portion configured to store a predetermined amount of the biogas and discharge the biogas exceeding the predetermined amount to the acid fermentation tank, and configured to block the opening with the biogas stored in the gas storage portion.
    Type: Application
    Filed: January 19, 2017
    Publication date: January 31, 2019
    Applicant: National Institute for Environmental Studies
    Inventors: Takashi ONODERA, Kazuaki SYUTSUBO, Motoyuki MIZUOCHI
  • Publication number: 20180338877
    Abstract: The purpose of the present disclosure is to provide a small-sized electric vehicle that is capable of assisting power of a wheelchair coupled to the small-sized electric vehicle, and that is capable of traveling alone in a state of being detached from the wheelchair. A folding vehicle 1 is capable of making a wheelchair 101 coupled by a coupling member 61 move in a self-traveling manner using a battery 14 and an in-wheel motor M etc. Furthermore, the folding vehicle 1 is capable of self-traveling by using the battery 14 and the in-wheel motor M etc. while in an independent state of not being coupled to the wheelchair 101.
    Type: Application
    Filed: August 7, 2018
    Publication date: November 29, 2018
    Applicants: TOYODA IRON WORKS CO., LTD., NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES, ACCURATE SYSTEMS INC.
    Inventors: Mitsutoshi KANO, Yoshinori KONDO, Junichi YASU
  • Patent number: 8765473
    Abstract: A basement membrane having a barrier function is formed by culturing alveolar epithelial cells or vascular endothelial cells on a fibrous collagen matrix coated with a polymer having a sugar chain that can localize a receptor that has an activity to accumulate a basement membrane component on the basal surface of the cells having an ability to form a basement membrane. A reconstructed artificial tissue is obtained by seeding and culturing desired homogeneous or heterogeneous cells on the basement membrane specimen constructed by the following process: (i) the cells having an ability to form a basement membrane adhered onto a support structure through a basement membrane are treated with a surface active agent; (ii) the lipid component of cells is lysed; (iii) the mixture of an alkaline solution and a protease inhibitor is used to lyse the protein remained on the surface of the basement membrane of the cells.
    Type: Grant
    Filed: November 15, 2006
    Date of Patent: July 1, 2014
    Assignees: Japan Science and Technology Agency, National Institute for Environmental Studies
    Inventor: Katsumi Mochitate
  • Patent number: 7972852
    Abstract: A reconstructed artificial tissue is obtained by seeding and culturing desired homogeneous or heterogeneous cells having an ability to form a basement membrane on the basement membrane specimen constructed by the following process: the cells having an ability to form a basement membrane adhered onto a support structure through a basement membrane are treated with a surface active agent; the lipid component of cells is lysed; the mixture of an alkaline solution and a protease inhibitor is used to lyse the protein remained on the surface of the basement membrane of the cells. A protein support structure is temporarily adhered to plastic surface through an adsorptive polymer by hydrophobic bonding, such as an alternating copolymer of methyl vinyl ether and maleic anhydride, which has a hydrophobic linear carbon skeleton and a functional group which can react with protein in a molecule and a basement membrane specimen or an artificial tissue is formed thereon.
    Type: Grant
    Filed: November 15, 2006
    Date of Patent: July 5, 2011
    Assignees: Japan Science and Technology Agency, National Institute for Environmental Studies
    Inventor: Katsumi Mochitate
  • Patent number: 7525086
    Abstract: An in-spray glow discharge ionization method and apparatus are provided The in-spray glow discharge ionization apparatus has a supply port supplying a fluid containing a compound to be measured, a gas blowing port which surrounds the supply port and which blows a gas exhibiting Penning effect to nebulize the fluid supplied from the supply port, a ground-side discharge electrode provided at a generation port at which the nebulized flow is generated, and a voltage application-side discharge electrode which is disposed in the traveling direction of the nebulized flow and opposed to the ground-side discharge electrode. In this in-spray glow discharge ionization method, while the fluid is nebulized by a spray gas (1), components of the compound to be measured which constitutes the fluid are ionized by the excited spray gas (1) exhibiting Penning effect, so that measurement is performed by a mass spectrometer.
    Type: Grant
    Filed: November 21, 2003
    Date of Patent: April 28, 2009
    Assignees: Japan Science and Technology Agency, National Institute for Environmental Studies
    Inventor: Shigeru Suzuki
  • Patent number: 7399634
    Abstract: The object of the present invention is to provide a method for preparing a basement membrane comprising extracellular matrices having a function to control morphology, differentiation, proliferation, motility, function expression and the like of cells. This basement membrane is formed by culturing cells having an ability to form a basement membrane on a fibrous collagen matrix coated with a polymer having a sugar chain. This sugar chain coat can localize a receptor which has an activity to accumulate a basement membrane component on the basal surface of the cells.
    Type: Grant
    Filed: March 25, 2004
    Date of Patent: July 15, 2008
    Assignees: Japan Science and Technology Agency, National Institute for Environmental Studies
    Inventor: Katsumi Mochitate
  • Publication number: 20060263878
    Abstract: The present invention provides a cell culture substrate which effectively adsorbs to a cell culture substrate such as a well, which is excellent in cell adhesion reproducibility, and whose surface is coated with a hydrophobic binding-adsorptive polymer and an immobilized preparation of cell adhesion proteins or peptides which effectively binds to the cell culture substrate and which is excellent in cell adhesion reproducibility, and further, an artificial tissue prepared by seeding and culturing cells on the immobilized preparation of cell adhesion peptides.
    Type: Application
    Filed: March 24, 2004
    Publication date: November 23, 2006
    Applicants: NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES, Katsumi Mochitate
    Inventor: Katsumi Mochitate
  • Patent number: 6099731
    Abstract: A process for treating a water containing chlorinated organic compounds, wherein a microorganism is introduced from a degradation tank to a separation tank and an energy source is supplied to the microorganism separated in the separation tank. A water-treatment apparatus having a structure capable of continuously introducing water to be treated through a degradation tank, a separation tank and a reactivation tank. A loss of the energy source such as methane or propane discharged from the separation tank can be reduced. A water-treatment apparatus having a degradation tank and a separation tank, wherein a microorganism can be cultivated in the separation tank. The reactivation tank can be omitted, whereby the entire size of the apparatus can be reduced.
    Type: Grant
    Filed: September 11, 1998
    Date of Patent: August 8, 2000
    Assignees: Ebara Research Co., Ltd., Japan as represented by Director General of Agency of National Institute for Environmental Studies
    Inventors: Hiroo Uchiyama, Osami Yagi, Masayoshi Kitagawa, Tatsuo Shimomura, Takeshi Hasegawa
  • Patent number: 5232855
    Abstract: The present invention provides an axenic mass culture apparatus comprising a light source for illuminating an entire surface of a culture liquid in a culture tank. According to the present invention, it is possible to achieve a light-illuminating type high-speed axenic mass culture of biotic cells.
    Type: Grant
    Filed: September 21, 1992
    Date of Patent: August 3, 1993
    Assignee: National Institute for Environmental Studies
    Inventors: Masataka Watanabe, Kunio Kohata