Abstract: A method of culturing bovine embryos by culturing said bovine embryos in a medium containing thiol compound, and also a method of transporting bovine embryos by transporting said bovine embryos in a medium containing thiol compound are disclosed. According to the present invention, it is possible to culture bovine embryos up to blastocyst stage efficiently in the medium containing low molecular thiol compound without in vivo culture or coculture using feeder cells. Also when low molecular thiol compound is added to the transportation medium for bovine embryos, almost 100% embryos are still viable after long distant transportation.

Abstract: There is provided a method of introducing exogenous genes into cultured cells or fertilized eggs, characterized in that each exogenous gene is introduced into chromosomal DNA in a cultured cell or fertilized egg under a condition where the exogenous gene is placed between insulators so as to ensure an insulated environment, such that the introduced exogenous gene will not be affected by any influence from adjacent genes in the cultured cell or fertilized egg.

Abstract: Cryopreservation of primordial germ cells and germ cells, in which primordial germ cells or germ cells are separated from bird embryos before meiosis and are then suspended and frozen in a freezing medium containing a cryoprotective agent is useful for the conservation of species of birds, for the protection of rare species which are in danger of extinction and also for the production of transgenic animals, the production of novel animals having gene markers on a cellular level, the production of animals useful as disease models, the production of useful substances by genetic engineering, and the development of test methods for toxicity to germ-line cells.

Abstract: A first solution containing Tris-HCl, MgCl.sub.2, a homopolymer of 2-acrylamide-2-methylpropanesulfonate (AMPS) and agarose is applied to a filter having alkaline phosphatase to be detected. A second solution containing Tris-HCl, MgCl.sub.2, homopolymer of AMPS, 5-bromo-4-chloroindoxyl phosphate, nitroblue tetrazolium and agarose is applied onto a film formed by the first solution.

Abstract: A fertilized ovum is recovered from an upper portion of a magnum of an oviduct of a dead hen 60 to 80 minutes after oviposition. The fertilized ovum is placed in a container containing a thin albumen. A yolk of the ovum is covered with a sheet of gauze, and the ovum is cultured up to the blastoderm stage (stage X) in the container which is covered with a sheet of plastic film that is held by a rubber band.

Abstract: Colors of sections of several beef carcasses are measured and the colors are classified into a predetermined number of grades of tone. Colored silicon plastic sol is poured in a glass cell for each grade, and the color of each plastic sol is matched with the classified beef color. The plastic sol is poured into a female mold having an irregular surface resembling a surface of a section of a beef, and the plastic is taken out from the female mold when it becomes gel.

Abstract: Resin pieces having a fat color corresponding to fat meats are molded and placed in a female mold. Resin having a lean meat color is pured in the female mold, and solidified resin is taken out from the mold.

Abstract: A system for managing milking-cows in stanchion stool for milking by which a cow is situated on a floor with her body being fixed in a standing position by supporting members and her neck being restricted in vertical movement by means of stoppers, with teatcups rising on the floor between the cow legs and fitted to her teats. The teatcups are individually set in predetermined positions, according to the peculiar teat positions of the cow.