Abstract: The present invention relates to a porcine bivalent subunit vaccine composition in a single dose. The porcine bivalent subunit vaccine composition includes porcine bivalent antigen, CpG adjuvant and a dual phase adjuvant. The porcine bivalent antigen consists of a classical swine fever virus (CSFV)-E2 recombinant protein and a porcine circovirus type 2 (PCV2)-ORF2 recombinant protein, both of which are produced by a mammalian cell expression system. The porcine bivalent subunit vaccine composition in a single dose can confer effectively immune protection against CSFV and PCV2 via a single vaccination without boost vaccination.

Abstract: The present disclosure relates to a mammalian cell line and a method for producing soluble E2 recombinant antigen of classical swine fever virus using the same. A mammalian cell expression system with different cell passage numbers can stably express a large amount of soluble CSFV-E2 recombinant protein, effectively reducing the production cost of CSFV-E2 recombinant protein, and then applied to the production of CSFV-E2 protein subunit vaccines.

Abstract: The present invention relates to an in vitro immune synapse system and a method of in vitro evaluating immune response using the same. The in vitro immune synapse system includes antigen-presenting cells (APCs) and at least one cell type of several specific T cell subtypes isolated from peripheral blood mononuclear cells (PBMCs), all of which is from a same individual of pigs. When a test sample is co-cultured in the in vitro immune synapse system for a given period, it can be determined that the test sample is immunogenic, immunostimulatory or not according to the immunization-related changes of these cells, thereby potentially replacing some kinds of animal experimentation.

Abstract: A holder provided for mounting of a lathe cutter includes a carrier, a first joint seat, a second joint seat, a limit rod and a clamp member. The first and second joint seats are disposed on a first and second base of the carrier respectively. The lathe cutter is mounted between the first and second joint seats. The limit rod is inserted into a through hole of the lathe cutter, and a first and second ends of the limit rod are coupled to the first and second joint seats respectively. The clamp member is disposed on the limit rod. The clamp member and the second joint seat are provided to clamp the lathe cutter to separate the lathe cutter from the first and second bases.

Abstract: A heat assisted flip chip bonding apparatus includes a semiconductor assembly having a substrate and a chip, a heating source and a press and cover assembly having a cover element and press elements. The chip is disposed above the substrate and includes conductors which contact with conductive pads on the substrate. The heating source is provided to emit a heated light which illuminates the chip via an opening of the cover element. The press elements are located between the cover element and the semiconductor assembly and each includes an elastic unit and a pressing unit. Both ends of the elastic unit are connected to the cover element and the pressing unit respectively, and the pressing unit is provided to press a back surface of the chip.

Abstract: Provided herein is Bacillus amyloliquefaciens strain PMB05 used in the field of plant disease control. Inoculation of plants with the strain can increase immune responses induced by detection of fungi and/or bacteria in plant, for example, increase the production of ROS and deposition of callose. Also, the strain has the effect of promoting plant growth. Therefore, the strain and its culture filtrate of the present invention can boost plant disease resistance and promote plant growth, which can be extensively used to control plant diseases and have the potential of turning into a commodified biological control agent.

Abstract: The present invention relates to a bacteriocin-producing Paenibocillus ehimensis strain NPUST-1, which can intensify the growth and immune response of aquaculture organisms. Also, the strain can increase the survival rate of aquaculture organisms after pathogen infection. A bacteriocin produced by the Paenibocillus ehimensis strain NPUST-1, Peocin, is a starvation/stationary phase protection protein, which has antimicrobial activity against various pathogen including aquaculture pathogens, foodborne pathogens and clinical pathogens. The bacteriocin Peocin is a starvation/stationary phase protection protein having antimicrobial activity found for the first time.

Abstract: The present invention relates to a bacteriocin-producing Paenibocillus ehimensis strain NPUST-1, which can intensify the growth and immune response of aquaculture organisms. Also, the strain can increase the survival rate of aquaculture organisms after pathogen infection. A bacteriocin produced by the Paenibocillus ehimensis strain NPUST-1, Peocin, is a starvation/stationary phase protection protein, which has antimicrobial activity against various pathogen including aquaculture pathogens, foodborne pathogens and clinical pathogens. The bacteriocin Peocin is a starvation/stationary phase protection protein having antimicrobial activity found for the first time.

Abstract: An inflatable planting device including a storage tank, a cultivation tank, a field planting tray and a plurality of planting frames is provided. The storage tank has a first air bag. The cultivation tank has a second air bag, and is located on the storage tank. The field planting tray has a mesh air bag defining a plurality of plant holes, and the field planting tray is located on the cultivation tank. The planting frames have a third air bag, and are connected to a periphery of the storage tank, the cultivation tank and the field planting tray through a plurality of connecting holes, where the connecting holes connect the first air bag, the second air bag, the mesh air bag and the third air bag to make the storage tank, the cultivation tank, the field planting tray and the planting frames to form an integrated connection.

Abstract: This invention provides a cell line of M2C macrophage and its applications. The cell line is derived from monocytes isolated from bone marrows and peripheral blood. The monocytes were differentiated into M2 macrophage by macrophage colony-stimulating factor (M-CSF), and then the polarization of M2C macrophage was induced by baicalin. The MERTK, PTX3, and PD-L1 expression level of the M2C macrophage are high and promote phagocytosis. Hence it can be applied to cell therapy or biological agents of immune regulation. Also, the macrophage-conditioned medium and wound dressing prepared on the M2C cell have the effects of enhancing fibroblast proliferation and angiogenesis, which can improve wound healing in medical use, and can be applied to skin care product for skin repair and rejuvenation.

Abstract: An isolated nucleic acid includes a sequence selected from the group consisting of the following: (i) a first nucleotide sequence of SEQ ID NO.: 7, SEQ ID NO.: 3, SEQ ID NO.: 9, or SEQ ID NO.: 5; (ii) a second nucleotide sequence encoding the amino acid sequence of SEQ ID NO.: 6, SEQ ID NO.: 4, SEQ ID NO.: 8, or SEQ ID NO.: 10; and (iii) a third nucleotide sequence complementary to the first nucleotide sequence or the second nucleotide sequence.

Abstract: The present invention relates to a nucleic acid-based adjuvant and a porcine vaccine composition including the same. The nucleic acid-based adjuvant, which is a nucleic acid fragment consisting of a sequence listing as SEQ ID NO: 1, can be used as the nucleic acid-based adjuvant of a vaccine composition, thereby elevating the protective ability of the porcine vaccine composition.

Abstract: An intelligent construction cone includes a light pervious body rotatable relative to a body. A rotating device is mounted to the light pervious body and driven by a driving device to rotate the light pervious body. A distance detector sends a distance signal to a controller coupled to the driving device. The distance detector is jointly rotatable with the light pervious body to eliminate detection dead angle. A warning device is coupled to the controller and generates a warning message responsive to the distance signal. A lighting device is coupled to the controller and provides illumination. A light sensor and a humidity sensor are coupled to the controller and detect environmental brightness and environmental humidity, respectively. The warning message and intensity of the illumination are adjusted according to a distance between an object and the distance detector, the environmental brightness, and the environmental humidity.

Abstract: The invention discloses a ventilation system including an inner, an outer tube and an air intake and exhaust apparatus. The inner tube has one end extending in a radial direction, wherein an air outlet and an air outlet are formed on the inner tube. An exhaust channel is formed between the inner and outer tubes, and the supporting member is disposed in the exhaust channel. The air intake and exhaust apparatus comprises an elevating member, a plurality of driving members and a cover plate. The elevating member has a connection block. The driving members are made of expandable material. The cover plate is rotatably coupled with the inner tube and has a connection portion. The connection portion abuts against the connection block when the driving members are not expanding. The connection portion of the cover plate disengages from the connection block when the driving members are expanding.

Abstract: A lined sleeve for tube welding is in the form of an inner tube including a first protruded portion and a second protruded portion. The first protruded portion and the second protruded portion are respectively received in two ends respectively of two tubes to be welded together. The inner tube further includes a recessed portion between the first protruded portion and the second protruded portion.

Abstract: A silver-containing antiseptic welding flux for stainless steel includes 0.1-0.5 wt % of silver, 30-54 wt % of silicon dioxide, 20-40 wt % of titanium dioxide, 10-20 wt % of chromium oxide, 5-20 wt % of molybdenum oxide, 5-10 wt % of molybdenum sulfide, and 5-10 wt % of halide.

Abstract: A use of Sargassum Cristaefolium extract in preparing a pharmaceutical or a food having a blood lipid regulation function is provided in the disclosure. The Sargassum Cristaefolium extract is obtained by performing a hot extraction process on Sargassum Cristaefolium.

Abstract: A cell/particle analyzing device includes a light-emitting unit, a light-diverting unit, a first receiving unit and a second receiving unit. The light-emitting unit generates a first light beam. The light-diverting unit is connected to the light-emitting unit and has an input end, a bidirectional transceiving end and an output end. The input end receives the first light beam generated by the light-emitting unit. The bidirectional transceiving end transmits the first light beam generated by the light-emitting unit and receives a second light beam. The output end outputs the second light beam. The first receiving unit is connected to the output end of the light-diverting unit and receives the second light beam. The second receiving unit is aligned with the bidirectional transceiving end.

Abstract: A plant imitating device includes a body having a compartment and an opening in communication with the compartment. A driving unit is mounted in the compartment and includes a transmission member. A plant imitating unit is mounted in the compartment and includes an end facing the opening of the body. The plant imitating unit is engaged with the transmission member by a mounting portion. The plant imitating unit is movable relative to the transmission member. A control unit is electrically connected to the driving unit. The control unit controls the driving unit to cause the end of the plant imitating unit to move beyond the body via the opening. Thus, the end of the plant imitating unit gradually extends beyond the opening to imitate the growth of a plant.

Abstract: An environment control apparatus for cultivating plants includes a casing having a bottom with first and second holes. A thermoelectric cooling module is mounted in the first hole and includes first and second sides. The first side faces the casing. A temperature control pipe includes two ends connected to a heat reservoir. The temperature control pipe includes a heat exchanging section in contact with the second side of the thermoelectric cooling module. A first power device is mounted on the temperature control pipe. An auxiliary heat pipe includes two ends connected to the heat reservoir. The auxiliary heat pipe includes a heat dissipating section located in the second hole. A processor is coupled to the thermoelectric cooling module and the first power device.