Patents Assigned to National Research Institute of Brewing
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Publication number: 20200040330Abstract: The present invention relates to a method of editing a filamentous fungal genome by direct introduction of a genome editing protein molecule or complex. The method includes three modes. In the first mode, a genome editing protein molecule or complex for a target gene is directly introduced into a cell of an Aspergillus fungus, to edit a gene in the Aspergillus fungal genome. In the second mode, a genome editing protein molecule or complex for a target region in a filamentous fungal genome, and a desired DNA fragment, are directly introduced into a filamentous fungal cell, to knock-in the DNA fragment to a desired target site in the filamentous fungal genome. In the third mode, genome editing protein molecules or complexes for plural target genes are directly introduced into a filamentous fungal cell, to carry out simultaneous editing of the plural genes in the filamentous fungal genome.Type: ApplicationFiled: August 25, 2017Publication date: February 6, 2020Applicants: NATIONAL RESEARCH INSTITUTE OF BREWING, FASMAC CO., LTD.Inventors: Kazuhiro IWASHITA, Ken ODA, Kohei SHIMAMOTO, Yusaku WADA
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Patent number: 9040257Abstract: The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERM BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.Type: GrantFiled: April 23, 2013Date of Patent: May 26, 2015Assignees: TOYOBO CO., LTD., NATIONAL RESEARCH INSTITUTE OF BREWINGInventors: Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani, Kazuo Masaki
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Publication number: 20150072379Abstract: The present invention provides a host for genetic recombination useful in the production of heterologous proteins in a large scale, by suppressing the production of extracellular polysaccharides in the basidiomycetous yeasts. Cryptococcus sp. S-2 D11 strain (FERN BP-11482) which is characterized in that the production of extracellular polysaccharides is suppressed as compared with the parent strain.Type: ApplicationFiled: April 23, 2013Publication date: March 12, 2015Applicants: TOYOBO CO., LTD., NATIONAL RESEARCH INSTITUTE OF BREWINGInventors: Yuu Utashima, Takahide Kishimoto, Shusaku Yanagidani, Kazuo Masaki
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Patent number: 7763444Abstract: It is intended to provide koji mold-origin phospholipase A2 and a DNA encoding it. Namely, phospholipase A2 comprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A2.Type: GrantFiled: June 23, 2009Date of Patent: July 27, 2010Assignees: National Institute of Technology and Evaluation, National Institute of Advanced Industrial Science and Technology, National Research Institute of BrewingInventors: Katsuhiko Kitamoto, Manabu Arioka, Shotaro Yamaguchi, Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Publication number: 20090304859Abstract: It is intended to provide novel compositions usable in improving the flavor of an alcoholic beverage made from grapes typified by wine. Namely, a composition usable in improving the flavor of an alcoholic beverage made from grapes which contains the culture of a strain belonging to a genus Aspergillus, Penicillium, Rhizopus, Rhizomucor, Talaromyces, Mortierella, Cryptococcus, Microbacterium, Corynebacterium or Actinoplanes and being capable of producing diglycosidase.Type: ApplicationFiled: August 17, 2009Publication date: December 10, 2009Applicants: Amano Enzyme Inc., National Research Institute of BrewingInventors: Kazutaka Tsuruhami, Hitoshi Amano, Shigeharu Mori, Nami Goto, Isao Aramaki, Akiko Fujita
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Publication number: 20090263888Abstract: It is intended to provide koji mold-origin phospholipase A2 and a DNA encoding it. Namely, phospholipase A2 comprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A2.Type: ApplicationFiled: June 23, 2009Publication date: October 22, 2009Applicants: National Institute of Technology and Evaluation, Natinal Institute of Advanced Industrial Science and Technology, National Research Institute of BrewingInventors: Katsuhiko Kitamoto, Manabu Arioka, Shotaro Yamaguchi, Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Patent number: 7348170Abstract: Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.Type: GrantFiled: December 25, 2002Date of Patent: March 25, 2008Assignees: Amano Enzyme Inc., National Institute of Advanced Industrial Science and Technology, National Institute of Technology and Evaluation, National Research Institute of BrewingInventors: Kensuke Yuuki, Atsuki Toumoto, Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Hisahara
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Patent number: 7186540Abstract: A completely novel glutaminase is provided: (a) a protein consisting of an amino acid sequence represented by SEQ ID NO: 2, or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one or more amino acids, and possessing glutaminase activity. This protein is a novel glutaminase possessing excellent thermostability and the like.Type: GrantFiled: December 24, 2002Date of Patent: March 6, 2007Assignees: National Institute of Advanced Indusrtial Science and Technology, National Institute of Technology and Evaluation, National Research Institute of BrewingInventors: Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara, Kotaro Ito, Kenichiro Matsushima, Yasuji Koyama
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Publication number: 20070004003Abstract: It is intended to provide koji mold-origin phospholipase A2 and a DNA encoding it. Namely, phospholipase A2 comprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A2.Type: ApplicationFiled: March 2, 2004Publication date: January 4, 2007Applicants: National Institute of Technology and Evaluation, National Institute of Advanced Industrial Science and Technology, National Research Institute BrewingInventors: Katsuhiko Kitamoto, Manabu Arioka, Shotaro Yamaguchi, Masayuki Machida, Keletsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Publication number: 20050287634Abstract: The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-?-1,3-glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade ?-1,3-glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or DNA encoding novel enzymes having ?-1,3-glucanase activity (exo-, or endo-?-1,3-glucanase), a recombinant expression vector comprising them, a microorganism having the recombinant expression vector, the novel enzymes having ?-1,3-glucanase activity, and a method for the production of said enzymes.Type: ApplicationFiled: March 22, 2005Publication date: December 29, 2005Applicants: National Institute of advanced Industrial Science and Technology, National Institute of Technology and Evaluation, National Research Institute of BrewingInventors: Masayuki Machida, Motoaki Sano, Misao Sunagawa, Tasuku Nakajima, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara