Abstract: The present invention discloses a portable pupil detection device with multiband stimulating light and infrared illumination, which comprises at least one pupil detection module for detecting the variation of the pupil of the testee. Each pupil detection module includes an illuminating light source emitting invisible light beams; a stimulating light source emitting visible light beams; at least one beamsplitter corresponding to the illuminating light source, the stimulating light source and an eye of the testee, cooperating with the illuminating light source and the eye to form a first optical path, and cooperating with the stimulating light source and the eye to form a second optical path; a lens corresponding to the beamsplitter and receiving the invisible light beams sent out by the beamsplitter to form an image of the eye; and an imaging element receiving the image of the eye from the lens.