Abstract: A Cas9 expression plasmid, a genome editing system and a genome editing method for Escherichia coli are provided. The Cas9 expression plasmid includes a tracrRNA sequence, a Cas9 gene and a chloramphenicol resistance gene (CmR). The Cas9 expression plasmid is applied to CRISPR/Cas-coupled ?-red recombineering system for editing genomes of E. coli with high efficiency.
Type:
Grant
Filed:
April 24, 2017
Date of Patent:
June 5, 2018
Assignees:
NATIONAL TSING HUA UNIVERISTY, CHANG CHUN PLASTICS CO., LTD, CHANG CHUN PETROCHEMICAL CO., LTD
Abstract: An inductor includes: a substrate; an insulator layer; a conductive coil; and a permeability-enhancing film of a multi-layer structure. The multi-layer structure includes at least one repeating unit that has at least two layers. The two layers exhibit an exchange-coupling effect and include a first ferromagnetic layer of a first ferromagnetic material and an exchange-coupling layer.
Type:
Application
Filed:
January 4, 2008
Publication date:
January 8, 2009
Applicant:
NATIONAL TSING HUA UNIVERISTY
Inventors:
Chih-Huang Lai, Ruo-Fan Jiang, Nazmun Nahar Shams, Chao-Chien Chiang
Abstract: The invention discloses the biodegradable nanoparticle for use in nanoparticle projectile bombardment as a carrier for administering proteins or peptides to an animal subject. The nanoparticles are composed of positively charged shell substrate, negatively charged core substrate with encapsulated bioactive agents of proteins or peptides.
Type:
Grant
Filed:
April 17, 2006
Date of Patent:
November 11, 2008
Assignees:
GP Medical, Inc., National Tsing Hua Univeristy
Inventors:
Hsing-Wen Sung, Mei-Chin Chen, Po-Wei Lee, Hosheng Tu