Abstract: The invention relates to a method for directing the self-assembly of a gene or gene assembly having three and preferably six or more fragments in a directionally and spatially ordered fashion to produce a gene, gene vector or large nucleic acid molecule. The method can be used to create libraries, such as combinatorial libraries. In another embodiment of the invention a vector is described for the incorporation and screeming of endogenous mouse promoter elements for the identification of cell-specific promoters.
Type:
Grant
Filed:
August 26, 1999
Date of Patent:
June 25, 2002
Assignee:
Nature Technology Corp
Inventors:
Clague P. Hodgson, Mary Ann Zink, Guoping Xu