Abstract: Compositions, systems, and methods are disclosed for preparing and utilizing arrays, such as single-analyte arrays containing a plurality of fiducial elements with random spatial distributions. Arrays may be prepared with pluralities of fiducial elements comprising optically active or passive moieties. Arrays containing random spatial distributions of fiducial elements may be utilized for various array-based processes that require spatial information.
Type:
Grant
Filed:
March 29, 2023
Date of Patent:
September 17, 2024
Assignee:
Nautilus Subsidiary, Inc.
Inventors:
Maryam Jouzi, Torri Elise Rinker, Pengyu Hao, Vadim Lobanov, Ryan Kempston Seghers, Daniel Horner, Pierre Indermuhle, David Stern, Ezra Van Gelder, Kevin Chen
Abstract: Compositions, systems, and methods for the display of analytes such as biomolecules are described. Display of analytes is achieved by coupling of the analytes to displaying molecules that are configured to associate with surfaces or interfaces. Arrays of analytes may be formed from the described systems for utilization in assays and other methods.
Type:
Grant
Filed:
January 18, 2024
Date of Patent:
August 27, 2024
Assignee:
Nautilus Subsidiary, Inc.
Inventors:
Tural Aksel, Stephen Hendricks, Elvis Ikwa, Pierre Indermuhle, Sadie Ingle, Christina Inman, Parag Mallick, Torri Elise Rinker, Steven Tan
Abstract: Methods and systems are provided herein for selecting an affinity reagent which binds a desired peptide epitope in a plurality of sequence contexts. The method relies on obtaining a peptide library, each peptide having the sequence ?X?, wherein X is the desired peptide epitope, wherein each of ? and ? comprise an amino acid, using the peptide library to select an affinity reagent.
Type:
Grant
Filed:
November 20, 2019
Date of Patent:
May 28, 2024
Assignee:
Nautilus Subsidiary, Inc.
Inventors:
Joshua S. Klein, Jarrett Egertson, Tracy Chan, Sonal Tonapi, Parag Mallick, Minyong Chung, Abdullah Ozer, Elliott Sorelle, David Stern
Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.
Type:
Grant
Filed:
March 7, 2023
Date of Patent:
May 28, 2024
Assignee:
Nautilus Subsidiary, Inc.
Inventors:
Tural Aksel, Torri Rinker, Markus Burns, Michael Dorwart, Rachel Galimidi, Dmitriy Gremyachinskiy, Stephen Hendricks, Elvis Ikwa, Gregory Kapp, Joshua Simon Klein, Julia Robinson, Cassandra Stawicki, Sonal Tonapi, Parag Mallick
Abstract: The present disclosure provides systems and methods for detecting components of an array of biological, chemical, or physical entities. In an aspect, the present disclosure provides a method for detecting an array of biological, chemical, or physical entities, comprising: (a) using one or more light sensing devices, acquiring pixel information from sites in an array, wherein the sites comprise biological, chemical, or physical entities that produce light; (b) processing the pixel information to identify a set of regions of interest (ROIs) corresponding to the sites in the array that produce the light; (c) classifying the pixel information for the ROIs into a categorical classification from among a plurality of distinct categorical classifications, thereby producing a plurality of pixel classifications; and (d) identifying one or more components of the array of biological, chemical, or physical entities based at least in part on the plurality of pixel classifications.
Type:
Grant
Filed:
June 10, 2021
Date of Patent:
March 19, 2024
Assignee:
Nautilus Subsidiary, Inc.
Inventors:
Jarrett D. Egertson, Vadim Lobanov, David Stern, Parag Mallick, Sujal M. Patel, Ryan K. Seghers
Abstract: Compositions, systems, and methods for the display of analytes such as biomolecules are described. Display of analytes is achieved by coupling of the analytes to displaying molecules that are configured to associate with surfaces or interfaces. Arrays of analytes may be formed from the described systems for utilization in assays and other methods.
Type:
Grant
Filed:
October 28, 2022
Date of Patent:
September 19, 2023
Assignee:
Nautilus Subsidiary, Inc.
Inventors:
Tural Aksel, Stephen Hendricks, Elvis Ikwa, Pierre Indermuhle, Sadie Ingle, Christina Inman, Parag Mallick, Torri Elise Rinker, Steven Tan
Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.
Type:
Grant
Filed:
November 10, 2021
Date of Patent:
July 4, 2023
Assignee:
Nautilus Subsidiary, Inc.
Inventors:
Tural Aksel, Torri Rinker, Markus Burns, Michael Dorwart, Rachel Galimidi, Dmitriy Gremyachinskiy, Stephen Hendricks, Elvis Ikwa, Gregory Kapp, Joshua Simon Klein, Julia Robinson, Cassandra Stawicki, Sonal Tonapi, Parag Mallick