Abstract: A method of isolating a protein from a mixture containing the protein, the method including providing an antibody immobilized on a solid support, which antibody is reactive with the protein complexed with a ligand and substantially unreactive with the protein not complexed with the ligand; contacting the mixture, in the presence of the ligand, with the immobilized antibody to bind the protein, complexed with the ligand, to the immobilized antibody to form an immune complex; and contacting the immune complex with a compound having a binding affinity for the ligand higher than the binding affinity of the protein for the ligand, to release the protein from the immobilized antibody.
Type:
Grant
Filed:
August 18, 1992
Date of Patent:
July 10, 2001
Assignee:
NE Medical Center Hospital, Inc.
Inventors:
Bruce E. Furie, Barbara C. Furie, Howard A. Liebman, Richard M. Lewis