Abstract: The present invention is directed to a multi-probe ablation simulation and guidance system and method for use in tissue ablation procedures. In use, the relative locations of a plurality of ablation probes capable of providing ablation energy are determined, and the effect of energy provided by the probes based on the determined locations is predicted to identify a simulated ablation volume. This simulated ablation volume is compared with a target tissue volume. The relative locations of the probes can be adjusted based on the comparison between the simulated ablation volume and the target tissue volume, and the predicted effect rerun until the simulated ablation volume encompasses the target tissue volume to be ablated and necrotized.