Abstract: Novel CE-phosphoramidites and CPG reagents have been synthesized from a serinol backbone. These reagents are useful to introduce functional groups or directly label oligonucleotides. The versatile serinol scaffold allows for labeling at any position (5? or 3? termini, or any internal position) during automated DNA synthesis. Multiple labels or functional groups can be achieved by repetitive coupling cycles. Optimal spacer arms and protected label moieties have been specially designed. Further, the natural 3-carbon atom internucleotide phosphate distance is retained when inserted internally.
Type:
Grant
Filed:
September 28, 2010
Date of Patent:
March 12, 2013
Assignees:
Glen Research Corporation, Nelson Biotechnologies, Inc.
Inventors:
Paul S. Nelson, Hugh Mackie, Andrew Murphy
Abstract: Novel CE-phosphoramidites and CPG reagents have been synthesized from a serinol backbone. These reagents are useful to introduce functional groups or directly label oligonucleotides. The versatile serinol scaffold allows for labeling at any position (5? or 3? termini, or any internal position) during automated DNA synthesis. Multiple labels or functional groups can be achieved by repetitive coupling cycles. Optimal spacer arms and protected label moieties have been specially designed. Further, the natural 3-carbon atom internucleotide phosphate distance is retained when inserted internally.
Type:
Application
Filed:
September 28, 2010
Publication date:
March 31, 2011
Applicants:
Nelson Biotechnologies, Inc., Glen Research Corporation
Inventors:
Paul S. Nelson, Hugh Mackie, Andrew Murphy