Patents Assigned to NEN Life Science Products, Inc.
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Patent number: 6593100Abstract: A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent comprising an inorganic salt, an organic compound having the formula wherein when X is B(OH)2, Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein tie heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from —OH, —COOH, —NH2, and —SH, and wherein R is selected from —OH, —COOH, —NH2, and —CH3; or mixtures of the inorganic salt and the organic compound.Type: GrantFiled: May 17, 2001Date of Patent: July 15, 2003Assignees: NEN Life Science Products, Inc., Washington UniversityInventors: Mark Norman Bobrow, Karl Edwin Adler, Kevin Aaron Roth
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Patent number: 6518036Abstract: A method for performing a peroxidase-based assay by reacting a peroxidase enzyme with a soluble organic p-hydroxyphenyl-containing compound having the formula: wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from —OH, —COOH, —NH2, and —SH, and wherein R is selected from —OH, —COOH, —NH2, and —CH3; and converting the soluble organic p-hydroxyphenyl-containing compound into a stable insoluble product which becomes highly fluorescent after illumination, upon excitation.Type: GrantFiled: March 16, 2000Date of Patent: February 11, 2003Assignees: Nen Life Science Products, Inc., Washington UniversityInventors: Mark Norman Bobrow, Kevin Aaron Roth
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Patent number: 6437141Abstract: A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: April 2, 2001Date of Patent: August 20, 2002Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow
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Patent number: 6316182Abstract: A method for detecting reverse transcriptase (RT), utilizing RT-catalyzed generation of cDNA complementary to an RNA template and detecting cDNA in a DNA hybridization reaction, is provided. Also provided is a method for detecting and/or quantitating drug resistance of reverse transcriptase in a sample and a method for evaluating ability of a compound to inhibit reverse transcriptase activity.Type: GrantFiled: July 14, 1992Date of Patent: November 13, 2001Assignee: Nen Life Science Products, Inc.Inventors: Richard Alfred Greene, Gerald Jospeh Litt
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Patent number: 6224644Abstract: A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: November 24, 1999Date of Patent: May 1, 2001Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow
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Patent number: 6204389Abstract: A cyanine dye having the formula wherein R1--R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: November 24, 1999Date of Patent: March 20, 2001Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow
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Patent number: 6197956Abstract: A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: November 24, 1999Date of Patent: March 6, 2001Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow
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Patent number: 6114350Abstract: A cyanine dye having the formula ##STR1## wherein R.sub.1 -R.sub.8 are each independently selected from a group consisting of hydrogen, C.sub.1 -C.sub.6 alkyl group, and C.sub.0 -C.sub.4 alkyl group having a hydrophilic substituent thereon. R.sub.11 and R.sub.12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: April 19, 1999Date of Patent: September 5, 2000Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow
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Patent number: 6066446Abstract: Disclosed is a member comprising an interactive material which is covalently bonded to a support body by a linker material. The member can be used as a part of an assay, and the support body may include a scintillator material.Type: GrantFiled: July 14, 1999Date of Patent: May 23, 2000Assignee: NEN Life Science Products, Inc.Inventors: Thomas R. Mullinax, Mark N. Bobrow, Michael E. Bembenek
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Patent number: 5972595Abstract: This invention relates to a hydrophobic layer formed on a solid support, the hydrophobic layer incorporating by hydrophobic interactions an amphipathic enzyme substrate labeled with a reporter on its hydrophilic region, and the use of the same to conduct various enzyme assays without extraction steps.Type: GrantFiled: December 16, 1998Date of Patent: October 26, 1999Assignee: NEN Life Science Products, Inc.Inventors: Patricia Kasila, Thomas R. Mullinax, Michael E. Bembenek, Mark N. Bobrow
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Patent number: 5843396Abstract: A stabilized composition comprising an organic compound labeled with a .beta.Type: GrantFiled: December 11, 1996Date of Patent: December 1, 1998Assignee: Nen Life Science Products, Inc.Inventors: Nadine Michele Loretta Gan, Gary Thomas Overmeyer, Douglas Roger Dennistoun Shaw