Abstract: A method of preparing neocyte-enriched blood is disclosed wherein a contained sample of whole blood is centrifuged at high speed so as to distribute the blood components in said chamber along a density continuum. Following the high-speed centrifuging step, the blood components in the first chamber are partitioned by transferring those components beyond a selected partition line in the first chamber from the spin axis to a second chamber so that the blood components in the first and second chambers can be used independently. Apparatus for effecting the separation and partitioning of the blood in a completely sterile environment is also disclosed.