Abstract: A method for producing an immune cell composition is disclosed. The method includes steps of: (a) culturing a tumor sample in a first container, (b) obtaining a first immune cell population from the cultured product obtained from (a); (c) seeding a second composition and a second immune cell population in a second container; (d) expending the second immune cell population to produce a third immune cell population; and (e) obtaining the third immune cell population from the expanded product obtained from (d) and producing the immune cell composition.