Abstract: A unidirectional fluid connection device, the device including an intermediate fluid storage capacity which is configured to temporarily store a given volume of a fluid, the device including an inlet channel for the fluid coming into the device and an outlet channel for the fluid going out of the device, the device further including a first one-way valve which is configured to provide one-way fluid communication from the inlet channel to the storage capacity, the device further including a second one-way valve which is configured to provide one-way fluid communication from the storage capacity to the outlet channel.

Abstract: A computer-assisted method for determining a microfluidic circuit configured to reproduce a neuron circuit, and comprising including the following steps: —obtaining a description of the neuron circuit, the description of the neuron circuit comprising a plurality of neuron populations and at least one neuron connection; —determining at least one first parameter for each node of a plurality of nodes of the microfluidic circuit, each node being associated with and configured to receive one neuron population among the plurality of neuron populations of the neuron circuit; —determining at least one second parameter for at least one connection of the microfluidic circuit, each connection being associated with and configured to receive a neuron connection of the at least one neuron connection of the neuron circuit; —determining the positioning of each node of the plurality of nodes and of each connection of the at least one connection.