Abstract: The invention relates to an improved method for the selection of hybridoma cells from a plurality of hybridoma cells for the generation of monoclonal antibodies as well as hybridoma cells together with suitable expression vectors by means of intracellular biotinylation and the use thereof.
Type:
Application
Filed:
July 16, 2020
Publication date:
June 15, 2023
Applicant:
NEW/ERA/MABS GMBH
Inventors:
Katja HANACK, Martin LISTEK, Burkhard MICHEE
Abstract: The invention relates, firstly, to a biomolecule-releasing cell characterised by a surface protein with an extracellularly exposed ligation peptide sequence for enzymatic conjugation of an adapter ligand, said adapter ligand being suitable for the indirect or direct coupling of a molecular catcher structure which has at least one specific binding site for the released biomolecules and which is at a distance from the specific binding site, secondly, to a method for selecting such a cell, and thirdly, to a means for this method comprising the surface protein that is characterised by an extracellularly exposable ligation peptide sequence for enzymatic conjugation of an adapter ligand, a nucleic acid coding for the surface protein, an expression vector for the nucleic acid, and a cell containing the expression vector.
Type:
Grant
Filed:
April 20, 2014
Date of Patent:
January 7, 2020
Assignee:
NEW / ERA / MABS GMBH
Inventors:
Martin Listek, Katja Hanack, Burkhard Micheel
Abstract: The invention relates, firstly, to a biomolecule-releasing cell characterised by a surface protein with an extracellularly exposed ligation peptide sequence for enzymatic conjugation of an adapter ligand, said adapter ligand being suitable for the indirect or direct coupling of a molecular catcher structure which has at least one specific binding site for the released biomolecules and which is at a distance from the specific binding site, secondly, to a method for selecting such a cell, and thirdly, to a means for this method comprising the surface protein that is characterised by an extracellularly exposable ligation peptide sequence for enzymatic conjugation of an adapter ligand, a nucleic acid coding for the surface protein, an expression vector for the nucleic acid, and a cell containing the expression vector.
Type:
Application
Filed:
April 20, 2014
Publication date:
February 16, 2017
Applicant:
NEW/ERA/MABS/GMBH
Inventors:
Martin LISTEK, Katja HANACK, Burkhard MICHEEL