Abstract: A new culture medium for identifying the presence of Salmonella inenterobacteria samples, especially faeces, contains two chromogenic enzyme substrates, one of which is a substrate for &agr;-D-galactosidase, for which Salmonella is positive. The other substrate is one for which Salmonella is negative such as &bgr;-D-galactosidase. The substrates are incorporated into an agar medium. Positive and negative results are found to be readily observable where one of the substrates is an esculetin, preferably a cyclohexenoesculetin compound in the presence of ferric ions, which produces a black color, and the other substrate is an indoxyl compound, for instance, a 5-bromo-4-chloro-3-indolyl compound which produces a green colored enzymic reaction product.