Abstract: A method of making a glow in the dark puff heat transfer material and a composition of glow in the dark puff heat transfer material are presented in this invention. Unlike many prior art heat transfer methods, the heat transfer method utilized in the present invention adheres to OSHA regulations 29 CFR 1910.1017. The process of making the glow in the dark puff heat transfer material comprises the steps of applying one or more layers of a specific glowing material between the colors layers. The composition comprises specific amounts of a glow clear base, glow puff material, translucent inks, and an effective amount of glowing material. A printable adhesive base may be added to the composition if the glowing material is not mixed with the glow clear base or with the glow puff material. The composition is printed on a paper substrate and cured through a conventional dryer.