Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.

Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.

Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.

Abstract: A composite polymer-coated sorbent with a bidisperse or oligodisperse distribution of pore sizes and having an at least partial coating on its surface, which coating comprises essentially polyanilines or derivatives of polyanilines and use thereof for the simultaneous separation and desalting of bio-macromolecules.

Abstract: A method for isolating RNA from RNA containing samples wherein the RNA containing sample is treated with at least one DNase and/or another enzyme like proteases or collagenases for purification of RNA in presence of a complex of ribonucleosides and the oxovanadyl ion which is capable of inhibiting RNases present in the RNA containing samples and removing the complex of ribonucleosides and the oxovanadyl ion prior to down-stream processing of the RNA of the RNA containing sample by contacting the sample of the forgoing step with a chelating agent immobilized to a support, the chelating agent having sufficient affinity to the complex of ribonucleosides and the oxovanadyl ion so that it binds to the chemical entity and the RNA containing sample is freed from the inhibitor.

Abstract: Use of a composite sorbent having an at least partial coating on a support, which coating comprises essentially polyanilines or derivatives of polyanilines for the separation, isolation, identification, purification and/or detection of biomolecules in particular nucleic acids, proteins, polysaccharides in analytical or preparative scale.