Abstract: Discussed herein are devices and methods for obtaining nucleotide sequence information from nucleic acid and nucleic acid samples. The device includes a fluidic channel that aids in manipulating a sample as the sample flows through various zones of the channel. Provided herein are methods for nucleic acid analysis that help to identify and filter out molecules that are in non-ideal conformations such as being folded, kinked, or overlapping with other molecules.
Abstract: Provided herein are methods and devices for obtaining nucleotide sequence information from nucleic acid and nucleic acid samples. The methods involve modifying and manipulating nucleic acids while in movement (flow) and without reliance on fixation, amplification or hybridization techniques. The methods and devices are sensitive enough to detect signal from and thus interrogate single nucleic acids on an individual basis rather than as a bulk population.