Abstract: Methods of preparing antibodies that recognize and bind three-dimensional epitopes of antigens are disclosed. The methods are particularly useful for preparing antibodies that bind the bioactive, three-dimensional amino terminus of parathyroid hormone. The antibodies so produced are used in diagnostic and therapeutic applications.

Abstract: Methods of preparing antibodies that recognize and bind three-dimensional epitopes of antigens are disclosed. The methods are particularly useful for preparing antibodies that bind the bioactive, three-dimensional amino terminus of parathyroid hormone. The antibodies so produced are used in diagnostic and therapeutic applications.

Abstract: A unique class of chemiluminescent labels containing biotin substitution that are suitable for chemiluminescent assays using inter alia a streptavidin and/or avidin conjugate. The chemiluminescent labels of the invention have the ability to bind to streptavidin and/or avidin per se or to streptavidin and/or avidin conjugated with an analyte. Label structures are disclosed that have hydrolytic stability to meet the most demanding commercial assay conditions. The invention encompasses conjugates containing associated versions of the labeling compounds, assays and kits for performing such assay utilizing the conjugates.

Abstract: A sampling device for use with an open necked container capable of holding a fluid material, comprises a hollow support member insertable into the container through the open necked portion thereof. The support member comprises a main body portion of a smaller diameter than the open neck of the container; a first end portion extends from the main body portion and is open to the ambient atmosphere through the open neck portion of the container. In one embodiment, elongated ribbed members extend from the main body portion and define a second end portion. The ribbed members form a generally rigid support structure. A dialysis tubing is fitted over the ribbed members and is closed at one end to define an open ended dialysis sac, the inside of which is directly accessible through the first end portion of the support structure.

Abstract: A method for the determination of a substance present in a sample, which comprises:(a) contacting a sample containing a substance with:(i) a first immunological binding partner to the substance, wherein the first immunological binding partner is a monoclonal antibody bound to biotin or a biotin-binding protein,(ii) a second immunological binding partner to the substance, wherein the second immunological binding partner is a detectably labeled region specific polyclonal antibody, and(iii) a biotin binding protein or biotin bound to a carrier;(b) incubating the components of step (a) for a period of time and under conditions sufficient to form an immune complex between the substance, the first immunological binding partner, the second immunological binding partner, and the carrier;(c) separating the carrier from the sample; and(d) determining the detectably labeled second immunological binding partner in either the sample or the carrier;wherein the reaction between the first immunological binding partner and th