Abstract: This invention provides a simple analysis method by a PCR method that simultaneously performs, in a single container, separating nucleic acid of pathogens contained in a tissue fragment and preparing a PCR buffer solution, and a kit used for the analysis method. A method for detecting a pathogen includes: (1) obtaining a liquid specimen mixture by adding a tissue fragment containing a pathogen to a PCR buffer solution containing a proteolytic enzyme; (2) heating the liquid specimen mixture at a first temperature; (3) further heating at a second temperature; (4) performing PCR by adding a portion of the liquid mixture obtained in (3) above to a solid composition for PCR reaction containing DNA polymerase and one or more kinds of PCR primer pair; and (5) detecting a PCR product generated in (4) above.
Type:
Application
Filed:
January 27, 2021
Publication date:
December 23, 2021
Applicants:
SHIMADZU CORPORATION, National University Corporation Tokyo Medical and Dental University, Nihon Techno Service Co., Ltd., National University Corporation Oita University, RIKEN, Toho University