Abstract: The present disclosure provides a method for extracting and separating flavonoids from Lindera aggregata leaves. The method includes: mixing Lindera aggregata leaves with an adsorbent, conducting elution with a matrix solid-phase dispersion (MSPD) extraction method, followed by concentration to obtain a Lindera aggregata leaf extract; conducting primary separation and secondary separation on the Lindera aggregata leaf extract by high-speed counter-current liquid chromatography (HSCCC), to separate quercetin-3-O-?-D-arabinofuranoside, a mixture of quercetin-3-O-?-D-glucoside and quercetin-5-O-?-D-glucoside, quercetin-3-O-rhamnopyranoside, and kaempferol-7-O-?-L-rhamnopyranoside; where a second solvent system used in the secondary separation includes ethyl acetate, n-butanol, an addictive and water, and the addictive includes cyclodextrin.