Abstract: The disclosure relates to the engineering of IL-35 cytokine polypeptides to achieve improved therapies for inflammatory diseases and conditions. Accordingly, aspects of the disclosure relate to a polypeptide comprising SEQ ID NO:2, wherein the polypeptide comprises one or more amino acid substitutions relative to SEQ ID NO:2, wherein the one or more amino acid substitutions comprise K23, R74, R195, R197, or combinations thereof. Further aspects relate to a polypeptide comprising SEQ ID NO:4, wherein the polypeptide comprises one or more amino acid substitutions relative to SEQ ID NO:4, wherein the one or more amino acid substitutions comprise C74. Further aspects relate to a polypeptide comprising SEQ ID NO:6, wherein the polypeptide comprises one or more amino acid substitutions relative to SEQ ID NO:6, wherein the one or more amino acid substitutions comprise C34.

Abstract: The present invention provides a sample breaking-up instrument and a method thereof, in which a pressing member is pressed by a centrifugal force against a sample within a cylindrical body provided with a filter member to thereby break-up the sample safely and efficiently. Provided is an instrument for breaking-up a sample, comprising: a cylindrical body having a through hole opening in both ends thereof; a filter member installed in one end of the cylindrical body within the through hole; and a pressing member to be operatively inserted into the hole of the cylindrical body from the other end thereof so as to be slidable therethrough in a sealed manner, wherein a force is exerted on the pressing member so that the pressing member presses the sample placed between the pressing member and the filter member against the filter member and thereby the sample is forced to pass through the filter member and is thus broken-up.

Abstract: The present invention provides a sample breaking-up instrument and a method thereof, in which a pressing member is pressed by a centrifugal force against a sample within a cylindrical body provided with a filter member to thereby break-up the sample safely and efficiently. Provided is an instrument for breaking-up a sample, comprising: a cylindrical body having a through hole opening in both ends thereof; a filter member installed in one end of said cylindrical body within said through hole; and a pressing member to be operatively inserted into said hole of said cylindrical body from the other end thereof so as to be slidable therethrough in a sealed manner, wherein a force is exerted on said pressing member so that said pressing member presses said sample placed between said pressing member and said filter member against said filter member and thereby said sample is forced to pass through said filter member and is thus broken-up.

Abstract: A water based photosensitive composition comprises a hydrolyzate of a mammal collagen and a photosensitizer serving to cross-link the hydrolyzate when exposed to an active light. The hydrolyzate has a number-average molecular weight, Mn, of 2,000 to 30,000 and an intrinsic viscosity, [.eta.], of 0.060 to 0.155 dl/g in a 0.15 mole citric acid buffer solution maintained at 40.degree.C. Also, the hydrolyzate is capable of maintaining the formability of the collagen fold. A photoresist pattern formed by using the composition exhibits a high resolution, a good dyeing property and a strong corrosion resistance.