Abstract: A flowcell device, including a flow pathway and an optical subassembly, has a flowcell body that is continuous with a sample being analyzed and a temperature controlled surface. The flowcell body can be disposed between a thermalplate, actively regulated by a thermoelectric cooler, and an insulating member. The flowcell device can be employed in a bioreactor monitoring system.

Abstract: A method for studying cell viability and protein aggregation involves establishing a Fabry Perot etalon signal within an optical spectroscopic feature, e.g., in the near infrared region. Protein aggregation and cell viability can be reflected by changes observed in the magnitude of the Fourier Transform peaks observed in the frequency or space domain associated with the contrast of the etalon. In short, the presence of viable cells and protein aggregates can degrade the etalon contrast of an etalon window. In some cases, the concentration of cells and monomeric protein can be measured as well.

Abstract: A device for monitoring a bioreactor is configured for in-situ analysis, e.g., by NIR, without the need for withdrawing a sample into a sample cell or into an ex-situ arrangement. The device can be inserted into a port of the bioreactor and provides a sample detection region defined by an optical element such as a lens and a photodetector. The electrical signal obtained from a photodetector that is part of the device can be directed to an analyzer via a detachable electrical connection.

Abstract: A device for monitoring a bioreactor is configured for in-situ analysis, e.g., by NIR, without the need for withdrawing a sample into a sample cell or into an ex-situ arrangement. The device can be inserted into a port of the bioreactor and provides a sample detection region defined by an optical element such as a lens and a photodetector. The electrical signal obtained from a photodetector that is part of the device can be directed to an analyzer via a detachable electrical connection.