Patents Assigned to Nitrate Elimination Co., Inc.
  • Systems and methods for enzymatic oxygen removal
    Patent number: 9187779
    Abstract: Systems and methods for oxygen removal from aqueous solutions are presented in which a bi-enzymatic reaction sequence recycles and depletes oxygen to extinction, preferably using an oxidase and a catalase as biocatalysts and a carbohydrate as co-substrate. Contemplated systems and methods are particularly advantageous in conjunction with electrochemical reaction systems in which oxygen would adversely interfere with the reaction system.
    Type: Grant
    Filed: February 21, 2012
    Date of Patent: November 17, 2015
    Assignee: The Nitrate Elimination Co., Inc.
    Inventors: Nicolas Plumere, Wilbur H. Campbell, Ellen R. Campbell
  • Simplified eukaryotic nitrate reductase
    Patent number: 7262038
    Abstract: The invention provides modification to the polynucleotide coding sequence for Pichia angusta NAD (P)H: nitrate reductase [YNaR1; GenBank accession number Z49110], which has Enzyme Commission number 1.7.1.2 (formerly EC 1.6.6.2), yielding the polynucleotide coding sequence for simplified eukaryotic nitrate reductase (S-NaR1). The invention also provides a method for recombinant expression of said polynucleotide code in the cells of the methylotrophic yeast Pichia pastoris to produce the polypeptide for S-NaR1, which binds the host-produced molybdenum-molybdopterin cofactor and intracellularly forms catalytically active, nitrate-reducing enzyme as small and stable multimeric proteins. The invention also provides a method for rapid and high-yielding purification of S-NaR1 by utilizing the hexa-histidine sequence at the carboxyl-terminus of said polypeptide for immobilized metal affinity chromatography.
    Type: Grant
    Filed: December 10, 2002
    Date of Patent: August 28, 2007
    Assignee: Nitrate Elimination Co., Inc.
    Inventors: Wilbur H Campbell, Guillaume G Barbier, Ellen R Campbell
  • Simplified eukaryotic nitrate reductase
    Publication number: 20060211084
    Abstract: The invention provides modification to the polynucleotide coding sequence for Pichia angusta NAD (P)H: nitrate reductase [YNaR1; GenBank accession number Z49110], which has Enzyme Commission number 1.7.1.2 (formerly EC 1.6.6.2), yielding the polynucleotide coding sequence for simplified eukaryotic nitrate reductase (S-NaR1). The invention also provides a method for recombinant expression of said polynucleotide code in the cells of the methylotrophic yeast Pichia pastoris to produce the polypeptide for S-NaR1, which binds the host-produced molybdenum-molybdopterin cofactor and intracellularly forms catalytically active, nitrate-reducing enzyme as small and stable multimeric proteins. The invention also provides a method for rapid and high-yielding purification of S-NaR1 by utilizing the hexa-histidine sequence at the carboxyl-terminus of said polypeptide for immobilized metal affinity chromatography.
    Type: Application
    Filed: December 10, 2002
    Publication date: September 21, 2006
    Applicant: Th Nitrate Elimination Co., Inc.
    Inventors: Wilbur Campbell, Guillaume Barbier, Ellen Campbell