Abstract: Provided herein is a novel epitope that can be used as a tag in methods for rapid and effective characterization, purification, and subcellular localization of polypeptides of interest, which comprise the tag. The tag is specifically recognized by an epitope specific antibody, which can be used to detect, capture, quantify, and/or purify polypeptides of interest that are tagged with the epitope. Also provided is novel epitope specific antibody.

Abstract: Provided herein is a novel epitope that can be used as a tag in methods for rapid and effective characterization, purification, and subcellular localization of polypeptides of interest, which comprise the tag. The tag is specifically recognized by an epitope specific antibody, which can be used to detect, capture, quantify, and/or purify polypeptides of interest that are tagged with the epitope. Also provided is novel epitope specific antibody.

Abstract: The invention relates to an injectable biocompatible composition based on a polymeric support as well as to a method for producing it, which composition which comprises at least one hydrophilic polymer, wherein the polymer is polymerizable in situ to form a gel, and wherein the hydrophilic polymer is crosslinkable serum albumin or crosslinkable serum protein. The composition can be used in the restoration, the reconstruction, and/or the replacement of tissues and/or organs, or as a drug release implant in mammals. The composition is particularly suitable for treating cartilage disorders of a diseased or injured articular site in a mammal.

Abstract: Provided herein is a novel epitope that can be used as a tag in methods for rapid and effective characterization, purification, and subcellular localization of polypeptides of interest, which comprise the tag. The tag is specifically recognized by an epitope specific antibody, which can be used to detect, capture, quantify, and/or purify polypeptides of interest that are tagged with the epitope. Also provided is novel epitope specific antibody.

Abstract: A method of detecting biomolecules present in a complex biological sample includes a) separating the biological sample into fractions according to at least one physical property of the biomolecules; and b) specifically detecting biomolecules present in the fractions using at least one solid-phase-based, immunological detection method including immobilizing the biomolecules from the individual fractions on microsphere populations specific for each fraction and distinguishable from one another.

Abstract: The present invention relates to a biomaterial for cell or tissue culture, based on a polymeric carrier, which contains at least one crosslinkable hydrophilic polymer. The polymer is functionalized with groups that are selected from maleimide, vinylsulfonic, acrylate, alkyl halide, azirine, pyridyl, thionitrobenzene acid groups, or arylating groups. The invention relates further to a method of production of said biomaterial, and the use of particular functionalizing groups for the production of a biomaterial for the cultivation of tissue and/or cells. The biomaterial can have biofactors that exert a particular action on cells.

Abstract: Provided herein is a novel epitope that can be used as a tag in methods for rapid and effective characterization, purification, and subcellular localization of polypeptides of interest, which comprise the tag. The tag is specifically recognized by an epitope specific antibody, which can be used to detect, capture, quantify, and/or purify polypeptides of interest that are tagged with the epitope. Also provided is novel epitope specific antibody.

Abstract: In a method for automated determination of the relative position (x/y/z) between a first hole (27) on a first microsystem component (11), which is preferably provided with a first channel (44) opening in the first hole (27), and at least one second hole (29) on a second microsystem component (12), which is preferably provided with a second channel (45) opening in the second hole (29), the two microsystem components (11, 12) lie in a liquid medium (41) at least in the region (25, 26) of the holes (27, 29). Under the supervision of a control device (15) controlled by a computer (22), the first and second microsystem components (11, 12) are displaced relative to one another into different relative positions (x/y/z). Electrical signals (37) are delivered to one of the two microsystem components (12, 12) and are recorded on the other of the two microsystem components (11, 12) as measurement values (38) which depend on the relative position of the two microsystem components (11, 12) with respect to one another.

Abstract: The present invention provides an apparatus and a method to assemble organotypical tissues which can then be perfused and investigated under preferably physiological conditions.

Abstract: In a method for transferring nanostructures into a substrate, the following order of steps is used: decorating a substrate with nanomaterials (13), etching the substrate (10), applying a coating (15), removing the nanomaterials (13), and etching the substrate (10).

Abstract: The present invention relates to a method for inhibiting and/or preventing angiogenesis, the method comprising the step of administering a biocompatible composition, which is polymerizable to a hydrogel-forming material and which is based on a hydrophilic polymer, for inhibiting and/or preventing angiogenesis or endothelial cell proliferation, wherein the hydrophilic polymer is crosslinkable serum albumin or crosslinkable serum protein, in a subject in need of being treated. The invention furthermore relates to a method for inhibiting and/or preventing angiogenesis or endothelial cell proliferation in a subject in need thereof, comprising the step of administering a polymerized hydrogel-material, which has been obtained by polymerizing a serum-albumin- or serum-protein-based composition.

Abstract: A microfluidic system (12) for assembling and subsequently investigating complex cell arrangements has a three-dimensional microstructure (11) in which the cell culture is assembled, cultivated and investigated. At least two microchannel segments (16, 17) through which the microstructure (11) can be perfused from outside with a medium (21, 22) run in the microstructure (11), whereby the microchannel segments (16, 17) run at least in sections approximately parallel or equidistant to one another. The two microchannel segments (16, 17) are separated from one another by a wall structure (25) in which at least one aperture (26) connecting the two microchannel segments (16, 17) is provided. An electrode arrangement (27) is provided in or on the microstructure (11) in order to generate an inhomogeneous electric field (28) in the region of the at least one aperture (26).

Abstract: A method for analyzing proteins makes use of an array of first capture molecules which are specific for peptide epitopes. The proteins to be analyzed or a protein mixture containing the proteins to be analyzed is degraded to peptide fragments corresponding to the peptide epitopes, after which the array of capture molecules is incubated with the peptide fragments. The peptide fragments bound to the capture molecules are then detected.

Abstract: A method for analyzing proteins makes use of an array of first capture molecules which are specific for peptide epitopes. The proteins to be analyzed or a protein mixture containing the proteins to be analyzed is degraded to peptide fragments corresponding to the peptide epitopes, after which the array of capture molecules is incubated with the peptide fragments. The peptide fragments bound to the capture molecules are then detected.

Abstract: A method for analyzing proteins makes use of an array of first capture molecules which are specific for peptide epitopes. The proteins to be analyzed or a protein mixture containing the proteins to be analyzed is degraded to peptide fragments corresponding to the peptide epitopes, after which the array of capture molecules is incubated with the peptide fragments. The peptide fragments bound to the capture molecules are then detected.

Abstract: A pair of measuring electrodes comprising a first and a second, preferably in each case sheet-like electrode comprises an insulation layer arranged between said electrodes. One or more nanopores, which extend through said insulation layer as far as said first electrode, the surface of which is at least partially uncovered by said nanopores, are provided in each second electrode. The invention also describes a biosensor comprising a pair of measuring electrodes of this type, an electrochemical cell comprising a biosensor of this type and a process for producing said pair of measuring electrodes.

Abstract: The invention relates to a method for coating a support plate for carrying out functional tests on biological cells, to a support plate for carrying out functional tests on biological cells and to the use of corresponding support plates for carrying out functional tests on biological cells.

Abstract: A method and a device are used for carrying out measurements on cells located in a liquid environment. Each cell is positioned with an underside of its membrane on a surface having a channel running through it. A negative pressure is established to aspirate the cells. Each cell is electrically scanned via at least one electrode which is spaced apart from the cell. The negative pressure is preferably established in a pulse-like manner to rupture the membrane in such a way that the cell interior enclosed by the membrane is connected to the channel.